| ABSTRACT Difficulty in obtaining abundant sporulation in culture of many species of Cercospora may be the limiting factor for studies of biology, systematics, and inoculation of the genus. Therefore, it is necessary to understand the nutritional and environmental requirements that influence mycelial growth, sporulation and germination. As it is difficult to obtain conidia of Cercospora coffeicola in vitro, different temperatures (17, 22, 27, and 32 °C) and light intensities (80, 160, 240, and 320 μmol m-2 s-1) were evaluated to optimize pathogen sporulation and assess favorable conditions for spore germination, aiming for a strategy of disease control. The dark treatment (0 μmol m-2 s-1) was added for sporulation. A significant interaction was found... |
