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Selection of white-rot fungi to formulate complex and coated pellets for Reactive Orange 165 decolourization Electron. J. Biotechnol.
Elgueta,Sebastián; Rubilar,Olga; Lima,Nelson; Diez,M. Cristina.
Six strains of white-rot fungi isolated from southern Chile were evaluated for their ergosterol/biomass correlation and ligninolytic potential in solid medium to formulate pellets for Reactive Orange 165 (RO165) decolourization. The fungus Anthracophyllum discolor was selected to formulate complex pellets (fungal mycelium, sawdust, and activated carbon), coated pellets (complex pellet + alginate) and simple pellets (fungal mycelium). The activity of ligninolytic enzymes (laccase, manganese peroxidase, manganese-independent peroxidase, and lignin peroxidase) was evaluated in both the complex and coated pellets in modified Kirk medium, and the morphology of the pellets was studied using scanning electron microscopy (SEM). Complex pellets of A. discolor...
Tipo: Journal article Palavras-chave: Complex pellets; Ligninolytic enzymes; RO165 decolourization; White-rot fungi.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600008
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Application of MALDI-TOF MS for requalification of a Candida clinical isolates culture collection BJM
Lima-Neto,Reginaldo; Santos,Cledir; Lima,Nelson; Sampaio,Paula; Pais,Célia; Neves,Rejane P..
Microbial culture collections underpin biotechnology applications and are important resources for clinical microbiology by supplying reference strains and/or performing microbial identifications as a service. Proteomic profiles by MALDI-TOF MS have been used for Candida spp. identification in clinical laboratories and demonstrated to be a fast and reliable technique for the routine identification of pathogenic yeasts. The main aim of this study was to apply MALDI-TOF MS combined with classical phenotypic and molecular approaches to identify Candida clinical isolates preserved from 1 up to 52 years in a Brazilian culture collection and assess its value for the identification of yeasts preserved in this type of collections. Forty Candida spp. clinical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BRCs; Candida; Clinical yeasts; Culture collections; MALDI-TOF MS.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200020
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