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Induction of the acrosome reaction test to in vitro estimate embryo production in Nelore cattle Arq. Bras. Med. Vet. Zootec.
Costa,M.Z.; Oliveira,L.Z.; Resende,M.V.; Lucio,A.C.; Perini,A.P.; Miguel,M.C.V.; Lima,V.F.M.H..
The effectiveness of induction of the acrosome reaction (AR) test as a parameter to in vitro estimate embryo production (IVP) in Nelore breed and the AR pattern by the Trypan Blue/Giemsa (TB) stain were evaluated. Frozen semen samples from ten Nelore bulls were submitted to AR induction and were also evaluated for cleavage and blastocyst rates. The treatments utilized for AR induction were: control (TALP medium), TH (TALP medium + 10μg heparin), TL (TALP medium + 100μg lysophosphatidylcholine) and THL (TALP medium + 10μg heparin + 100μg lysophosphatidylcholine). Sperm acrosomal status and viability were evaluated by TB staining at 0 and after 4h incubation at 38°C. The results obtained for AR presented a significant difference (P<0.05) in the percentage...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Fertility; Semen; Acrosome reaction; Fertilization.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400001
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Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production Arq. Bras. Med. Vet. Zootec.
Lucio,A.C; Resende,M.V; Dernowseck-Meirelles,J.A.; Perini,A.P.; Oliveira,L.Z; Miguel,M.C.V; Carmo,A.S; Tomita,S.Y; Alves,B.C.A; Fazano,F.A.T.; Lima,V.F.M.H..
The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was performed in in vitro produced embryos. The results were validated by the sex ratio of in vitro produced embryos using PCR by Y- specific sequences present in bovine male genomic DNA. After determining genetic sex of in vitro produced embryos, the results showed difference (P<0.05) in deviation of sex ratio when comparing the control group (45.2% females) with the other spermatozoa selection procedures (60.6% females) (P<0.05). The sperm selection methods are...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Density gradient centrifugation; Embryo sex ratio; Percoll™; Swim-up.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300001
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