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| Linden,Rafael; Matte,Ursula. |
| Gene therapy attempts the insertion and expression of exogenous genetic material in cells for therapeutic purposes. Conceived in the 1960s, gene therapy reached its first clinical trial at the end of the 1980s and by December 2013 around 600 genuine open clinical trials of gene therapy were registered at NIH Clinical Trials Database. Here, we summarize the current efforts towards the development of gene therapy in Latin America. Our survey shows that the number of scientists involved in the development of gene therapy and DNA vaccines in Latin America is still very low. Higher levels of investment in this technology are necessary to boost the advancement of innovation and intellectual property in this field in a way that would ease both the social and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene therapy; Gene transfer; Gene delivery; Viral vector; South America. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200015 |
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| Tagliari,Carmela Farias da Silva; de Oliveira,Cáren Nunes; Vogel,Greice Meyer; da Silva,Patrícia Baptista; Linden,Rafael; Lazzaretti,Rosmeri Kuhmmer; Notti,Regina Kuhmmer; Sprinz,Eduardo; Mattevi,Vanessa Suñé. |
| Abstract HIV-infected individuals on chronic use of highly active antiretroviral therapy (HAART) are more likely to develop adipose tissue and metabolic disorders, such as lipodystrophy (LD) and metabolic syndrome (MetS). The development of these phenotypes is known to be multifactorial. Thus, variants in genes implicated in adipogenesis and lipid metabolism may increase susceptibility to LD and MetS. Sirtuin 1 (SIRT1) may influence the outcome of these disturbances due to its role in the regulation of transcription factors involved in energy regulation. Therefore, we genotyped four polymorphisms located in SIRT1 (rs2273773 T>C, rs12413112 G>A, rs7895833 A>G, rs12049646 T>C) in 832 HIV-infected patients receiving HAART by real-time polymerase... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV; Lipodystrophy; Metabolic syndrome; Sirtuin 1. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100105 |
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| Luz,Ana Júlia; Poeta,Júlia; Linden,Rafael; Antunes,Marina Venzon; Caminha,Luiza Isola; Sprinz,Eduardo. |
| OBJECTIVE: To evaluate the factors associated with plasma concentrations of atazanavir (ATV) in a cohort of well-controlled HIV infected subjects (undetectable viremia). Design: Cross-sectional study where 69 subjects were consecutively enrolled between April and November, 2011. METHODS: Patients had to be on atazanavir for at least six months, undetectable viral load for a period equal to or longer than 12 months, T CD4+ lymphocyte count higher than 200 cells/mm³, and aged between 18 years and 70 years old. Exclusion criteria were pregnancy, any neurologic disease, active opportunistic disease, hepatitis or cancer. Atazanavir plasma levels were measured by ultra-performance liquid chromatography. RESULTS AND DISCUSSION: Overall, 54 patients (mean age of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atazanavir; Plasma levels; Drug concentration; Ritonavir; Lipid profile. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600006 |
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| Castilhos,Juliana Kich; Dillenburg,Thaís Luise; Antunes,Marina Venzon; Scribel,Letícia; Zavascki,Alexandre Prehn; Linden,Rafael; Verza,Simone Gasparin. |
| Polymyxins are a group of antibacterial substances and have remained the drugs of choice for treatment of resistant Gram-negative bacilli. Polymyxin B is administered by intravenous infusion and requires the reconstitution of lyophilized powder with 0.9% saline or 5% glucose solutions. To date, there is little information about polymyxin stability in different infusions solutions, especially at 40 ºC, a temperature that is recommended to study drug stability as it accelerates degradation reactions. Therefore, in this work an analytical method using LC-MS/MS was developed, validated and applied to determine the stability of polymyxin B diluted in 0.9% saline or 5% glucose solutions at 25 ºC and 40 ºC. The stability of polymyxin B solutions was evaluated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polymyxin; Stability; LC-MS/MS; Glucose solutions; Saline solutions. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100537 |
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