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	Provedor de dados Nature Precedings (2) Autor Anthony L. Salvagno (2) Lawrence J. Herskowitz (2) Linh N. Le (2) R. Andy Maloney (2) Steven J. Koch (2) Palavra-chave Biotechnology (2) Genetics & Genomics (2) Tipo do documento Manuscript (2) Ano 2010 (1) 2009 (1) País United Kingdom (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Proof of principle for shotgun DNA mapping by unzipping Nature Precedings Lawrence J. Herskowitz; Anthony L. Salvagno; R. Andy Maloney; Linh N. Le; Steven J. Koch. Ability to map polymerases and nucleosomes on chromatin is important for understanding the impact of chromatin remodeling on key cellular processes. Current methods (such as ChIP and ChIP-chip) have produced a wealth of information that demonstrates this importance, but key information is elusive in these ensemble methods. We’re pursuing a new single-molecule chromatin mapping method based on unzipping native chromatin molecules with optical tweezers. The first step we are taking towards this ability is shotgun DNA mapping (SDM). This is the ability to identify the genomic location of a random DNA fragment based on its naked DNA unzipping forces compared with simulated unzipping forces of a published genome. We show that ~32 separate... Tipo: Manuscript Palavras-chave: Biotechnology; Genetics & Genomics. Ano: 2009 URL: http://precedings.nature.com/documents/2808/version/1 Proof of principle for shotgun DNA mapping by unzipping Nature Precedings Lawrence J. Herskowitz; Anthony L. Salvagno; R. Andy Maloney; Linh N. Le; Steven J. Koch. Ability to map polymerases and nucleosomes on chromatin is important for understanding the impact of chromatin remodeling on key cellular processes. Current methods (such as ChIP and ChIP-chip) have produced a wealth of information that demonstrates this importance, but key information is elusive in these ensemble methods. We’re pursuing a new single-molecule chromatin mapping method based on unzipping native chromatin molecules with optical tweezers. The first step we are taking towards this ability is shotgun DNA mapping (SDM). This is the ability to identify the genomic location of a random DNA fragment based on its naked DNA unzipping forces compared with simulated unzipping forces of a published genome. We show that ~32 separate... Tipo: Manuscript Palavras-chave: Biotechnology; Genetics & Genomics. Ano: 2010 URL: http://precedings.nature.com/documents/2808/version/2 Registros recuperados: 2 Primeira ... 1 ... Última

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