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| Kaspar, Vojtech; Hulak, Martin; Kohlmann, Klaus; Vandeputte, Marc; Rodina, Marek; Gela, David; Linhart, Otomar. |
| Sperm competition occurs when sperm from different males compete for fertilization. The aim of this in vitro fertilization study was to compare the competitive success of five males using heterosperm with an equal number of sperm from each male and four different sperm/egg ratios (5,000, 10,000, 20,000 and 100,000 spermatozoa per egg) in order to better understand variations of competitive fertilization. The roles of sperm motility and velocity were studied. Fertilization and hatching rates of the 5 males studied, with 100,000 spermatozoa per egg, were between 23.7-94.8% and 23.7-92.2%, respectively. Sperm velocity and percentage of motile sperm ranged from 85.0 to 137.6 mu m s(-1) and 2.0 to 93.5% at 15 see post sperm activation, respectively. The... |
| Tipo: Text |
Palavras-chave: Paternity assignment; Artificial reproduction; Aquaculture. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6551.pdf |
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| Siddique, Mohammad A. Momin; Butts, Ian A. E.; Linhart, Otomar; Macias, Adrian D.; Fauvel, Christian. |
| Studying gamete biology can provide important information about a species fertilization strategy as well as their reproductive ecology. Currently, there is a lack of knowledge about how long sea bass Dicentrarchus labrax eggs can remain viable after being activated in seawater. The objectives of this study were to understand the effects of pre-incubation of fresh and overripe sea bass eggs in seawater and to determine the duration of egg receptivity. Pooled eggs (fresh and overripe) from four females were pre-incubated in seawater for 0 min (control), 0.5 min, 1 min, 3 min, 10 min and 30 min and then fertilized by pooled sperm from four males. The fresh eggs had a higher fertilization success than overripe eggs. Our results revealed a significant effect of... |
| Tipo: Text |
Palavras-chave: Egg longevity; Overripe eggs; Reproductive ecology; Mediterranean sea bass; Dicentrarchus labrax. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00279/39000/37580.pdf |
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