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Endo-beta-mannanase from the endosperm of seeds of Sesbania virgata (Cav.) Pers. (Leguminosae): purification, characterisation and its dual role in germination and early seedling growth Braz. J. Plant Physiol.
Lisboa,César Gustavo Serafim; Tonini,Patrícia Pinho; Tiné,Marco Aurélio Silva; Buckeridge,Marcos Silveira.
Galactomannans are storage cell wall polysaccharides present in seeds of some legumes. Their degradation is carried out by three hydrolases (alpha-galactosidase (EC 3.2.1.22), endo-beta-mannanase (EC 3.2.1.78) and ß-mannosidase (EC 3.2.1.25)). In the present study we purified and characterised an endo-beta-mannanase from seeds of Sesbania virgata and addressed its role in germination and seedling development. The polypeptide purified by Ion Exchange Chromatography and Affinity Chromatography on Sepharose-Concanavalin A, showed a pH optimum between 3.5 and 5 at 45ºC and high stability at pH 7.8. The low stability at pH 5 appears to be associated with isoelectric precipitation, in view of the pI of the enzyme being 4.5. The purified enzyme is a glycoprotein...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sesbania virgata; Endo-beta-mannanase; Galactomannan; Germination; Leguminosae.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200003
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