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	Provedor de dados BABT (1) Autor Li,Yaning (1) Liu,Daqun (1) Yan,Hongfei (1) Yang,Wenxiang (1) Zhang,Yan (1) Palavra-chave Chromosome walking (1) Nonspecific primer anchored PCR (1) Random primer (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2011 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última A nonspecific primer anchored PCR technique for chromosome walking BABT Zhang,Yan; Liu,Daqun; Yang,Wenxiang; Li,Yaning; Yan,Hongfei. A chromosome walking method was improved in this work. The new method was named as nonspecific primer anchored PCR (NPA-PCR). Nested gene specific primers were designed based on the known region and long random primer using degeneracy oligonucleotides for nonspecific anchoring. Annealing temperatures were varied to control the priming. Target sequences were obtained by PCR with random primer and gene-specific primer. Nonspecific sequence with long random primers at both ends formed stem loop structure due to inverted terminal repeats. The method was employed to isolate a gene with newly-isolated actinomycin producing strain Streptomyces setonii Z-L-22. A 0.77 kb fragment of actinomycin synthetase gene cluster was isolated from the strain. The fragments of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nonspecific primer anchored PCR; Chromosome walking; Random primer. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100013 Registros recuperados: 1 Primeira ... 1 ... Última

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