|
|
| Huo,Shengdong; Yuan,Qijian; Zhang,Taojie; Liu,Junlin; Abudureyimu,Ayimuguli; Yang,Jutian. |
| ABSTRACTThe objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens). The analysis showed that the level of expression of ER mRNA was greater in the pituitary gland tissue than in other glands during estrus. Immunofluorescence analyses showed that ER proteins were located in the pineal cells, synaptic ribbon, and synaptic spherules of the pineal gland. In the hypothalamus, ER proteins were located in the magnocellular and parvocellular neurons. The ER proteins were located in acidophilic cells and basophilic cells in the pituitary gland. In the ovary, ER proteins were present in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immunofluorescence; Real-time PCR. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982015001000350 |
| |
