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Zhao,Guiping; Zheng,Maiqing; Chen,Jilan; Wen,Jie; Wu,Chunmei; Li,Wenjuan; Liu,Libo; Zhang,Yuan. |
Avian leukosis virus subgroup J (ALV-J) is a new type of virus that mainly induces myeloid leukosis (ML) in chickens. To further elucidate the pathogenesis of ALV-J infection and tumor development, expression profiles from the bone marrow tissue of 15 infected and 18 non-infected birds from a local-breed poultry-farm under naturally infected conditions, were analyzed by suppression-subtractive hybridization. The birds were diagnosed as ML+ (or ML-) by specific ALV-J detection methods, involving serological tests for antigens and antibodies, and RT-PCR to detect viral RNA. A total of 59 partial gene sequences were revealed by differential screening of 496 forward and 384 reverse subtracted cDNA clones. Of these, 22 identified genes, including 8 up-regulated... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian leukosis virus subgroup J; Chicken; Transferring gene; MHC genes; Hemoglobin gene. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100009 |
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