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| Yao,Xiaoqin; Liu,Qing; Han,Chao. |
| Ultraviolet-B (UV-B) radiation and nitrogen are expected to increase simultaneously with future changes in global climate. In this study, growth and photosynthetic responses of Picea asperata seedlings to enhanced UV-B and to nitrogen supply were studied. The experimental design included two levels of UV-B treatments (ambient UV-B, 11.02 kJ m-2 d-1; enhanced UV-B, 14.33 kJ m-2 d-1) and two nitrogen levels (0; 20 g N m-2) to determine whether nitrogen can alleviate the negative impacts of enhanced UV-B on seedling growth and photosynthesis. Enhanced UV-B significantly inhibited plant growth and impaired net photosynthetic rate, stomatal conductance, transpiration rate, the light-saturated assimilation rate, assimilation capacity, light compensation point,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Growth; Gas exchange; Nitrogen; Picea asperata; Photosynthesis; UV-B. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202008000100002 |
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| Liu,Zixuan; Zhang,Jizhou; Fan,Hongqiong; Yin,Ruofeng; Zheng,Zhong; Xu,Qian; Liu,Qing; He,Haiting; Peng,Xiaofan; Wang,XinXin; Li,Xiaokun; Xiao,Yechen. |
| Background Overexpression or mutated activation of Fibroblast growth factor receptor 3 (FGFR3) is involved in the pathogenesis of many tumors. More and more studies focus on the potential usage of therapeutic antibodies against FGFR3. Results In this study, a novel single-chain Fv (ScFv) against FGFR3 was prepared and characterized. To achieve the soluble expression, ScFv was fused with Sumo (Small ubiquitin-related modifier) by polymerase chain reaction (PCR), and cloned into pET-20b. The recombinant bacteria were induced by 0.5 mM Isopropyl-ß-d-thiogalactopyranoside (IPTG) for 16 h at 20°C, and the supernatant liquid of Sumo-ScFv was harvested and purified by Ni-NTA chromatography. After being cleaved by the Sumo protease, the recombinant ScFv was... |
| Tipo: Journal article |
Palavras-chave: Escherichia coli; Fibroblast growth factor receptor 3; Single-chain Fv antibody; Soluble expression; Sumo tag. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400008 |
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