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	Provedor de dados Electron. J. Biotechnol. (1) Autor Liu,Shiwang (1) Mao,Jianwei (1) Mei,Lehe (1) Wu,Yuanfeng (1) Palavra-chave Broccoli seed (1) Central composite design (1) Plackett-Burman design (1) Production (1) Sulforaphane (1) Tipo do documento Journal article (1) Ano 2013 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Studies on statistical optimization of sulforaphane production from broccoli seed Electron. J. Biotechnol. Wu,Yuanfeng; Mao,Jianwei; Mei,Lehe; Liu,Shiwang. Background: Natural sulforaphane (SF) has been of increasing interest for nutraceutical and pharmaceutical industries due to its anti-cancer effect. The main objective of the present work was to optimize the production of SF from broccoli seed using response surface methodology. Results: Three major factors (hydrolysis time, water volume and ethyl acetate volume) were screened out through Plackett-Burman (PB) factorial design. The methods of steepest ascent combined with central composite design (CCD) were employed for optimization of the SF production process. The optimal extraction conditions for SF production were a hydrolysis time of 13 min, a hydrolysis volume/weight ratio of 2.9:1 (v/g) and an extraction volume/weight ratio of 17.5:1 (v/g). The... Tipo: Journal article Palavras-chave: Broccoli seed; Central composite design; Plackett-Burman design; Production; Sulforaphane. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600001 Registros recuperados: 1 Primeira ... 1 ... Última

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