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| Wang,W; Liu,Z; Qian,W; Fang,Y; Liang,S. |
| Peptide toxins are usually highly bridged proteins with multipairs of intrachain disulfide bonds. Analysis of disulfide connectivity is an important facet of protein structure determination. In this paper, we successfully assigned the disulfide linkage of two novel peptide toxins, called HNTX-III and HNTX-IV, isolated from the venom of Ornithoctonus hainana spider. Both peptides are useful inhibitors of TTX-sensitive voltage-gated sodium channels and are composed of six cysteine residues that form three disulfide bonds, respectively. Firstly, the peptides were partially reduced by tris(2-carboxyethyl)-phosphine (TCEP) in 0.1 M citrate buffer containing 6 M guanidine-HCl at 40° C for ten minutes. Subsequently, the partially reduced intermediates containing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Disulfide bonds; TCEP; Partial reduction; HNTX-III; HNTX-IV. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200009 |
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| Qian,J; Wang,Z; Liu,Z; Kuang,W; Busso,CA. |
| Understanding the changes in the total bud bank, and its contribution to community regeneration, in response to grassland enclosure to livestock grazing, is crucial for grassland management. Despite its importance, the contribution of the total bud bank and that of different bud types to community regeneration as a whole have been rarely explored. The vegetative offspring recruited from different bud types was investigated in grasslands having different enclosure durations to livestock grazing on a typical steppe of Inner Mongolia, China. Total vegetative offspring density was significantly higher (p<0.05) under continuous grazing than in fenced grasslands, but no significant changes were found among plots with different enclosure durations to livestock... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grazing; Bud banks; Vegetative regeneration; Reproduction ecology; Restoration ecology. |
| Ano: 2014 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572014000200024 |
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| Shi,H; Deng,X; Deng,Q; Liu,Z; Liu,N. |
| ABSTRACT This study aimed to investigate the effect of Lactobacillus plantarum DPP8 and Lactobacillus acidophilus C7282 in feed supplementation on growth performance, Salmonella invasion, inflammation, and mediating signaling in broilers infected with Salmonella Typhimurium (S. Typhimurium). A total of 240 broilers at day old were randomly allocated into four groups, orally infected with S. Typhimurium and supplemented with individual or combined Lactobacilli DPP8 and C7282 at doses of 0 (control), 1010 (individual), or 2.0 × 1010 (combination) cfu/kg of diet for 21 d. The results showed that supplementing Lactobacilli improved (p<0.05) feed intake and body weight gain and decreased (p<0.05) S. Typhimurium load in the caecum, harder gland, spleen and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: IFNγ; Interleukin; Salmonella Typhimurium load; TNFα. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100302 |
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| Mahfuz,S; Song,H; Wei,J; Chen,M; Zhen,D; Nahar,J; Liu,Z. |
| ABSTRACT The possibility of using mushroom (Flammulina velutipes) stem waste (FVW) in layer diets was explored in this study. A total of 180, 40-week-old ISA Brown laying hens were randomly assigned to 5 groups, with 6 replications of 6 birds for each group. Layers were fed a standard basal diet as control; antibiotic (0.05% flavomycin); 2% FVW; 4%FVW; and 6% FVW. No significant differences (p>0.05) were observed on hen daily egg production, egg weight, egg mass, unmarketable eggs, feed intake, feed conversion ratio, and live weight among experimental groups. Haugh unit was found higher (p<0.05) in FVW fed groups than in antibiotic fed groups. Shape index, shell weight, shell thickness, yolk weight, yolk index, yolk weight and albumen weight were not... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apparent nutrient retention; Calcium utilization; Egg quality; Laying performance; Mushroom waste. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400717 |
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| Liu,Z; Xue,J; Luo,Y; Wang,Q; Zhong,H; Liang,M; Wang,C. |
| ABSTRACT The objective of the study was to investigate the mechanism by which dietary energy concentration regulates laying performance in geese. Eighty 558-day-old female Sichuan White geese were randomly allotted to two dietary treatments, each treatment was fed 1 of 2 experimental diets containing 10.00 (deficient) or 11.80MJ/kg metabolizable energy (sufficient) for 30 days. Laying performance, hormone concentration and gene expressions in hypothalamus-pituitary-gonadal axis were examined in geese. Birds fed the sufficient-energy diet had significantly higher average egg weight, daily laying rate, and lower feed to egg ratio than those fed the deficient-energy (p<0.05). The birds fed sufficient-energy diet had higher concentration of serum insulin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Energy concentration; Laying geese; Reproductive hormone; Gene expression. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300306 |
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| Wu,Q; Liu,Z; Li,S; Jiao,C; Wang,Y; Wang,Y. |
| ABSTRACT The aim was to investigate the effect of glutamine (Gln) on broilers challenged with Salmonella Enteritidis. 240 1-day-old birds were divided into four groups in a completely randomized design, each of which included 6 replicates with 10 birds per replicate. Group I served as the unchallenged, untreated control (CON). All birds in groups II (SCC) - IV were challenged with 2.0 × 104 CFU/mL of S. Enteritidis. Birds in group III and IV were treated with 0.5% (Gln 1) and 1.0% (Gln 2), respectively, of Gln. The results indicated that S. Enteritidis infection led to a decrease in the average body weight at d 7, 14, and 21 (p<0.05). Chickens fed the Gln showed improved average body weights in comparison with the SCC group (p<0.05). At d 4, 7, 14,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutamine; Average body weight; Antioxidant function; Salmonella Enteritidis; Broiler. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400307 |
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| Liu,Z; Wu,Q; Jiao,C; Cheng,B; Zhu,D; Ma,Y; Li,Y; Li,W. |
| ABSTRACT The aim of this study was to investigate the effects of glutamine (Gln) on the intestinal mucosal structure and immune cells of broilers infected with Salmonella Enteritidis. 160 1-d-old commercial Arbor Acres (AA) broilers were randomly selected to receive one of four treatments, each of which had 5 replicates. Each replicate consisted of 8 chicks subjected to a 21-d feeding trial. Group I served as the unchallenged (CON). All birds in groups II (SCC) - IV were challenged with 2.0 × 104 CFU/mL of S. Enteritidis. The birds in groups III and IV were treated with 0.5% and 1.0% Gln. The results showed that S. Enteritidis infection led to a decrease in the relative length and weight, villus height:crypt depth (VH:CD) of the jejunum and ileum, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutamine; Intestinal villi; Mucosal immunity; Salmonella Enteritidis; Broiler. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000300309 |
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