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| Lopes,Ana Daniela; Scapim,Carlos Alberto; Machado,Maria de Fátima Pires da Silva; Mangolin,Claudete Aparecida; Silva,Tereza Aparecida; Cantagali,Liriana Belizário; Teixeira,Flávia França; Mora,Freddy. |
| Information on genetic diversity is essential to the characterization and utilization of germplasm. The genetic diversity of twenty-two sweet corn cultivars (seventeen open-pollinated varieties, OPV, and five hybrids, H) was investigated by applying simple sequence repeat markers. A total of 257 primers were tested, of which 160 were found to be usable in terms of high reproducibility for all the samples tested; 45 were polymorphic loci, of which 30 were used to assess the genetic diversity of sweet corn cultivars. We detected a total of 86 alleles using 30 microsatellite primers. The mean polymorphism was 82 %. The highest heterozygosity values (Ho = 0.20) were found in the PR030-Doce Flor da Serra and BR427 III OPVs, whereas the lowest values (0.14) were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Simple sequence repeats; Neighbor-joining; Maize germplasm. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000600513 |
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| Zaghi Júnior,Lienine Luiz; Lopes,Ana Daniela; Cordeiro,Fábio Aparecido; Colla,Itaruã Machri; Bertéli,Míria Benetati Delgado; Valle,Juliana Silveira do; Linde,Giani Andrea; Colauto,Nelson Barros. |
| Abstract Agaricus subrufescens is a basidiomycete which is studied because of its medicinal and gastronomic importance; however, less attention has been paid to its preservation. This study aimed to evaluate the effect of sucrose addition to substrate and cryotube on the viability of Agaricus subrufescens cryopreserved at -20 °C and at -75 °C for one and two years. Zero, 10% or 20% sucrose was added to potato dextrose agar or wheat grain. The mycelia were cryopreserved in the absence of cryoprotectant or with sucrose solutions at 15%, 30% or 45%. After one or two years at -75 °C or at -20 °C, mycelia were thawed and evaluated about viability, initial time of growth, colony diameter and genomic stability. Cryopreservation at -20 °C is not effective to keep... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Preservation; Freeze; Cereal grain; Monosaccharide; Agaricus blazei. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200370 |
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| Linde,Giani Andrea; Luciani,Alana; Lopes,Ana Daniela; Valle,Juliana Silveira do; Colauto,Nelson Barros. |
| Abstract Basidiomycetes have several biotechnological and industrial applications such as enzyme production, bioremediation, pharmaceutical and functional food production. Due to climatic features, the preservation of several basidiomycetes is threatened, and to guarantee the preservation of this genetic resource, the development of long-term preservation techniques is necessary once there is no universal protocol for the cryopreservation of basidiomycetes. Cryopreservation is a technique in which microorganisms are submitted to ultralow temperatures. Therefore, this study aimed to collect information on the main conditions for long-term cryopreservation of basidiomycetes in the last 20 years. Scientific articles on cryopreservation of basidiomycetes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Preservation; Substrate; Fungi; Mycelial viability; Cryoprotectant. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200220 |
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