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| Lu,Y.; Rong,C.Z.; Zhao,J.Y.; Lao,X.J.; Xie,L.; Li,S.; Qin,X.. |
| The shipment and storage conditions of clinical samples pose a major challenge to the detection accuracy of Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), and Ureaplasma urealyticum (UU) when using quantitative real-time polymerase chain reaction (qRT-PCR). The aim of the present study was to explore the influence of storage time at 4°C on the DNA of these pathogens and its effect on their detection by qRT-PCR. CT, NG, and UU positive genital swabs from 70 patients were collected, and DNA of all samples were extracted and divided into eight aliquots. One aliquot was immediately analyzed with qRT-PCR to assess the initial pathogen load, whereas the remaining samples were stored at 4°C and analyzed after 1, 2, 3, 7, 14, 21, and 28 days. No... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Storage time; Chlamydia trachomatis; Ureaplasma urealyticum; Neisseria gonorrhoeae; Stability; QRT-PCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001000701 |
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| Zhou,X.; Lv,W.; Zhang,W.; Ye,Y.; Li,Y.; Zhou,Q.; Zhang,J.; Xing,Q.; Lu,Y.; Zhang,L.; Wang,H.; Qin,W.; Tang,B.. |
| This meta-analysis compared the efficacy and safety of the contact force (CF)-sensing catheter and second-generation cryoballoon (CB) ablation for treating atrial fibrillation (AF). Six controlled clinical trials comparing ablation for AF using a CF-sensing catheter or second-generation CB were identified from PubMed, EMBASE, Cochrane Library, Wanfang Data, and China National Knowledge Infrastructure. The procedure duration was significantly lower in the CB group compared with that in the CF group [mean difference (MD)=29.4; 95%CI=17.84–40.96; P=0.01], whereas there was no difference between the groups for fluoroscopy duration (MD=0.59; 95%CI=–4.48–5.66; P=0.82). Moreover, there was no difference in the incidence of non-lethal complications (embolic event,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atrial fibrillation; Ablation; Contact force-sensing catheter; Second-generation cryoballoon; Meta-analysis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000900607 |
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