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	Provedor de dados BABT (1) Autor Barreto,André Silva (1) Luca,Michelle Jaccottet de (1) Souza,Tiago Tolentino de (1) Weber,Laura Isabel (1) Palavra-chave Cytochrome-b (1) Dentin (1) Dolphins (1) Mitochondrial DNA (1) Museum specimens (1) Tursiops (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2007 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Successful amplification of mitochondrial DNA from dentin of the bottlenose dolphin Tursiops truncatus BABT Weber,Laura Isabel; Luca,Michelle Jaccottet de; Barreto,André Silva; Souza,Tiago Tolentino de. Twenty-four teeth of Tursiops truncatus from the collection of the Marine Mammals Laboratory (FURG-RS) were analysed for their potential as DNA source for population genetic and taxonomic studies. All teeth were submitted to decontamination, grinding and demineralisation processes. Dentin powder was weighed for DNA extraction, which was evaluated by the amplification of two mitochondrial genes through PCR using universal primers. Cytochrome-b gene was amplified successfully. PCR products showed concentrations of 3-12 ng/µl for dentin powder, which was positively correlated to the quantity of dentin powder used for DNA extraction. To rule out contamination a SSCP was carried out including positive controls, and samples of humans involved in the analysis.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Dolphins; Tursiops; Museum specimens; Dentin; Mitochondrial DNA; Cytochrome-b. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100002 Registros recuperados: 1 Primeira ... 1 ... Última

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