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	Provedor de dados BJM (1) Autor He,Ying (1) Hou,Deng-Yong (1) Luo,Qun (1) Shen,Xian-Rong (1) Zhang,Jian-Guo (1) Palavra-chave Alkane hydroxylase (1) Biodegradation (1) Diesel oil (1) Escherichia coli DH5α (1) Expression (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2015 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última GPo1 alkB gene expression for improvement of the degradation of diesel oil by a bacterial consortium BJM Luo,Qun; He,Ying; Hou,Deng-Yong; Zhang,Jian-Guo; Shen,Xian-Rong. To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (alkB) gene of Pseudomonas putida GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 alkB plasmid was transformed into Escherichia coli DH5α. The AlkB protein was expressed by diesel oil induction and detected through SDS-polyacrylamide gel electrophoresis. The culture of the recombinant (pCom8-GPo1 alkB/E. coli DH5α) with the oil biodegradation bacterial consortium increased the degradation ratio of diesel oil at 24 h from 31% to 50%, and the facilitation rates were increased as the proportion of pCom8-GPo1 alkB/E. coli DH5α to the consortium increased. The results suggested that the expression of the GPo1... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkane hydroxylase; Expression; Escherichia coli DH5α; Biodegradation; Diesel oil. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300649 Registros recuperados: 1 Primeira ... 1 ... Última

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