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	Provedor de dados Bragantia (2) Scientia Agricola (1) PAB (1) Autor Müller,Gerd Walter (4) Nunes,William Mário de Carvalho (2) Zanutto,Carlos Alexandre (2) Corazza,Maria Júlia (1) Costa,Alessandra Tenório (1) Donadio,Luiz Carlos (1) Fadel,André Luiz (1) Oliveira,Carolina Rossi de (1) Parolin,Luiz Gustavo (1) Pio,Rose Mary (1) Sawazaki,Haiko Enok (1) Silva,Simone Rodrigues da (1) Sodek,Ladaslav (1) Stuchi,Eduardo Sanches (1) Mais... Palavra-chave Citrus sinensis (2) Cross-protection (2) Bud-union crease (1) Citros (1) Citrus (1) Citrus paradisi × Poncirus trifoliata (1) Identificação de espécies (1) Isoenzimas (1) Preimmunization (1) RFLP (1) Rootstock (1) SSCP (1) Single strand conformational polymorphism (1) Stem pitting symptoms (1) Mais... Tipo do documento Info:eu-repo/semantics/article (4) Ano 2019 (1) 2013 (1) 2010 (1) 1992 (1) País Brazil (4) Idioma Inglês (3) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Stability of Citrus tristeza virus protective isolates in field conditions PAB Costa,Alessandra Tenório; Nunes,William Mário de Carvalho; Zanutto,Carlos Alexandre; Müller,Gerd Walter. The objective of this work was to monitor the maintenance of Citrus tristeza virus (CTV) protective isolates stability in selected clones of 'Pêra' sweet orange (Citrus sinensis), preimmunized or naturally infected by the virus, after successive clonal propagations. The work was carried out in field conditions in the north of Paraná State, Brazil. Coat protein gene (CPG) analysis of 33 isolates collected from 16 clones of 'Pêra' sweet orange was performed using single strand conformational polymorphism (SSCP). Initially, the isolates were characterized by symptoms of stem pitting observed in clones. Then viral genome was extracted and used as template for the amplification of CPG by reverse transcription polimerase chain reaction (RTPCR). RTPCR products... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Cross-protection; Preimmunization; Single strand conformational polymorphism; Stem pitting symptoms. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700009 Evaluation of the protective capacity of new mild Citrus tristeza virus (CTV) isolates selected for a preimmunization program Scientia Agricola Zanutto,Carlos Alexandre; Corazza,Maria Júlia; Nunes,William Mário de Carvalho; Müller,Gerd Walter. The use of tolerant rootstocks and preimmunization has satisfactorily controlled losses associated with the Citrus tristeza virus (CTV). Several researchers have shown that CTV mild isolates that are selected in the same region where they are used are superior to isolates obtained from other areas. Thus, budwoods of 20 outstanding citrus trees were collected in north and northwestern Paraná state (Brazil) citrus-producing areas and established to be used in a preimmunization program. These budwoods were tested to evaluate the potential protection of the inherently present viral complex. Based on biological indexing and molecular characterization of the capsid protein gene by RFLP (restriction fragment length polymorphism, which indicated that the plants... Tipo: Info:eu-repo/semantics/article Palavras-chave: SSCP; RFLP; Cross-protection; Citrus. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000200009 Identificação de espécies de citros mediante polimorfismo enzimático Bragantia Sawazaki,Haiko Enok; Sodek,Ladaslav; Pio,Rose Mary; Müller,Gerd Walter. Estudou-se, mediante polimorfismo enzimático em gel de poliacrilamida, a variabilidade genética das espécies de laranja-doce (Citrus sinensis); laranja-azeda (C. aurantium); tangerinas clementina (C. clementina), sunki (C. sunki), cleópatra (C. reshni) e poncã (C. rsticulata); lima-da-pérsia (C. limettioides); limão-galego (C. aurantifolia); limão-cravo (C. limonia) e trifoliata (Poncirus trifoliata). Extratos de folhas foram analisados para as isoenzimas de malato deidrogenase (MDH), enzima málica (ME), leucino amino peptidase (LAP), glutamato oxaloacetato transaminase (GOT), fosfoglucoisomerase (PGI), fosfoglucomutase (PGM) e isocitrato deidrogenase (IDH). Verificou-se grande variabilidade genética interespecífica, porém nenhuma entre os cultivares de... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citros; Isoenzimas; Identificação de espécies. Ano: 1992 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87051992000200002 Compatibility and horticultural performance of Pera sweet orange clones grafted to Swingle citrumelo rootstock Bragantia Fadel,André Luiz; Stuchi,Eduardo Sanches; Silva,Simone Rodrigues da; Parolin,Luiz Gustavo; Oliveira,Carolina Rossi de; Müller,Gerd Walter; Donadio,Luiz Carlos. ABSTRACT Despite its interesting agricultural traits, Swingle citrumelo (Citrus paradisi Macfad. ‘Duncan’ × Poncirus trifoliata L. Raf.) rootstock exhibits severe incompatibility when grafted with Pera sweet orange [Citrus sinensis (L.) Osb.] scions, and this fact limits its use for propagation purposes. Nonetheless, the existence of certain Pera sweet orange clones that are compatible with Swingle citrumelo represents a substantial gain for the entire citrus industry. This study investigated the compatibility between seven Pera sweet orange clones and Swingle citrumelo rootstock, regarding the presence of a necrotic line at the graft union junction, the difference in diameter between rootstock and scion, the influence of clonal cleaning by micrografting,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Citrus paradisi × Poncirus trifoliata; Rootstock; Bud-union crease. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052019000400564 Registros recuperados: 4 Primeira ... 1 ... Última

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