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Detection of the nonsense mutation of OPA3 gene in Holstein Friesian cattle with dilated cardiomyopathy in Japan OAK
HORIUCHI, Noriyuki; KUMAGAI, Daishiro; MATSUMOTO, Kotaro; INOKUMA, Hisashi; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; 松本, 高太郎; 猪熊, 壽; 古岡, 秀文; 古林, 与志安.
Bovine dilated cardiomyopathy (DCM) is an autosomal recessive genetic disorder causing congestive heart failure and subsequent death. Recently, a nonsense mutation c.343C>T in the bovine optic atrophy 3 (OPA3) gene had been reported to cause the DCM in Holstein cattle in Switzerland. However, the mutation has not been confirmed in bovine DCM outside Switzerland. Nine Holstein Friesian cows that were macroscopically and histologically diagnosed with or suspected of DCM and 12 control cows kept in Japan were tested for the mutation. The mutation surrounding OPA3 DNA fragment was amplified by PCR and subjected to direct sequences. The homogeneous c.343C>T mutation was proved to occur in all the affected cows and not in the control cows. The present...
Palavras-chave: Cattle; Dilated cardiomyopathy; Nonsense mutation; OPA3 gene.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4208
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Survey on Tick-Borne Pathogens in Thoroughbred Horses in the Hidaka District, Hokkaido, Japan OAK
YBAÑEZ, Adrian Patalinghug; SATO, Fumio; NAMBO, Yasuo; FUKUI, Takashi; MASUZAWA, Toshiyuki; OHASHI, Norio; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; INOKUMA, Hisashi; 松本, 高太郎; 猪熊, 壽.
A total of 87 Thoroughbred horses and 10 ixodid ticks from a ranch in Hidaka district, Hokkaido were tested for tick-borne diseases. Using the indirect fluorescent antibody (IFA) method, 3.4, 92.0 and 97.7% of the horses showed antibody titers of ≥80 against Anaplasma phagocytophilum, Rickettsia helvetica, and Borrelia garinii, respectively. This is the first report of infection with the 3 pathogens in horses in Japan. Using PCR, DNAs from the peripheral blood of all horses were found negative with any Anaplasma, Rickettsia and Borrelia spp., while those from Haemaphysalis megaspinosa ticks were found positive for Anaplasma sp. closely related to A. phagocytophilum in Japan, and A. bovis. B. japonica was also detected in an H. flava tick for the first time.
Palavras-chave: Anaplasma sp.; Borrelia sp.; Rickettsia sp.; Thoroughbred horse; Tick-borne diseases.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3840
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Use of Contrast-Enhanced CT in the Diagnosis of Abscesses in Cattle OAK
LEE, Ki-Ja; KISHIMOTO, Miori; SHIMIZU, Junichiro; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; SASAKI, Naoki; ISHII, Mitsuo; INOKUMA, Hisashi; IWASAKI, Toshiroh; MIYAKE, Yoh-Ichi; YAMADA, Kazutaka; 古林, 与志安; 松本, 高太郎; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽; 山田, 一孝.
We report here the non-contrast and contrast-enhanced computed tomography performed in two calves with brain abscess and multiple pulmonary abscesses with pharyngeal abscess, respectively. Contrast-enhanced computed tomography was useful in the diagnosis of these diseases in both calves. The diseases were confirmed by histopathological examination.
Palavras-chave: Abscess; Cattle; Contrast-enhanced CT; Diagnosis.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3891
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A Clinical Case of Severe Anemia in a Sheep Coinfected with Mycoplasma ovis and 'Candidatus Mycoplasma haemovis' in Hokkaido, Japan OAK
TAGAWA, Michihito; TAKEUCHI, Toshihiko; FUJISAWA, Tetsuro; KONNO, Yasuhiro; YAMAMOTO, Shinji; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
A 2-year-old East Friesian sheep imported from Australia exhibited severe anemia after contagious pustular dermatitis in Hokkaido, Japan. Hemoplasma infection was confirmed in blood smears. Both Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’ were detected by PCR and sequence analyses. In the epidemiological analysis, dual pathogens were detected in 6 of 12 (50.0%) sheep imported from Australia with the infected ewe at the same time, 1 of 5 (20.0%) sheep introduced from a domestic farm in Hokkaido, and in 1 of 16 (6.3%) sheep from an epidemiologically unrelated ranch. It is the first clinical case of sheep to confirm coinfection of these pathogens in Japan.
Palavras-chave: 'Candidatus Mycoplasma haemovis'; Case report; Japan; Mycoplasma ovis; Sheep.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3582
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急性鼻出血を主訴とするホルスタイン種乳牛10症例における臨床所見の病態別比較 OAK
猪熊, 壽; 滄木, 孝弘; 松本, 高太郎; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; INOKUMA, Hisashi; AOKI, Takahiro; MATSUMOTO, Kotaro; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4145
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Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis OAK
MIURA, Saori; HORIUCHI, Noriyuki; MATSUMOTO, Kotaro; KOBAYASHI, Yoshiyasu; KAWAZU, Shin-ichiro; INOKUMA, Hisashi.
Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL) without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to 3 EcoRI and HindIII fragments in these 2 atypical EBL cattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. The probe also hybridized to a large number of EcoRI and HindIII fragments in 5 cattle with persistent leukosis. These results suggest that the detection of monoclonal integration of BLV provirus into the host genome may serve as a marker of monoclonal proliferation and malignancy in difficult to diagnose EBL cattle.
Palavras-chave: Atypical enzootic bovine leukosis; Diagnostic marker; Monoclonal integration.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4143
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心内膜炎の併発がみられた心室中隔欠損のホルスタイン種成乳牛の1症例 OAK
岩上, 慎哉; 新谷, 紗代; 髙橋, 英二; 松本, 高太郎; 古岡, 秀文; 猪熊, 壽; MATSUMOTO, Kotaro; FURUOKA, Hidefumi; INOKUMA, Hisashi.
心室中隔欠損とは胎生期に中隔の形成過程が障害され る先天性心奇形である[1、5-7]。牛では最も多く見られる 心奇形で、臨床症状は欠損孔の大きさと位置により様々 であり、一般的には発育不良やチアノーゼを呈するが、 軽度なものでは無症状のまま成長する場合もある[1、5-7]。 一方、心内膜炎は弁膜表面に細菌が付着して炎症性変化 が起こる疾患であり、牛では、ほとんどが疣贅性で、特 に三尖弁と肺動脈弁に生じやすいとされている[2-4、6、7]。 今回、肺動脈弁と三尖弁に心内膜炎を併発した心室中隔 欠損のホルスタイン種成乳牛の1症例に遭遇したので、 その概要を報告する。
Palavras-chave: 心内膜炎; 心室中隔欠損; ホルスタイン.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4008
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血清チミジンキナーゼ活性の測定により早期摘発した地方病性牛白血病罹患牛の1例 OAK
坂本, 礼央; 大林, 哲; 古林, 与志安; 松本, 高太郎; 石井, 三都夫; 猪熊, 壽; SAKAMOTO, Leo; OHBAYASHI, Tetsu; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; ISHII, Mitsuo; INOKUMA, Hisashi.
チミジンキナーゼはDNA合成に関わる酵素の一つであり,近年牛白血病の発症マーカーとして利用できることが報 告されている.今回,牛白血病ウイルス汚染農場において,本酵素の活性を測定することにより,牛白血病罹患牛の早期摘発を試みた.その結果,臨床症状は示さなかったものの,地方病性牛白血病に罹患していた10歳9カ月齢のホルスタイン種雌牛を摘発できた.牛白血病ウイルス汚染牛群における本酵素活性の測定が,地方病性牛白血病罹患牛の早期摘発に臨床上有用であることが本研究により示唆された. Thymidine kinase is an enzyme related to DNA synthesis. Recently,serum thymidine kinase (STK) activity has been reported as a useful marker of bovine leukosis. In an effort to achieve earlier detection of enzootic bovine leukosis (EBL),we measured STK activity in cows at a farm tainted by bovine leukemia virus (BLV). We found that a Holstein cow aged 10 years and 9 months without any clinical symptoms showed higher STK activity,and the cow was diagnosed as having EBL. Evaluation of STK activity in a herd contaminated...
Palavras-chave: 地方病性牛白血病; 早期摘発; 血清チミジンキナーゼ活性; Early case detection; Enzootic bovine leukosis; Serum thymidine kinase activity.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2942
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Detection of the New Ehrlichia Species Closely Related to Ehrlichia ewingii from Haemaphysalis longicornis in Yonaguni Island, Okinawa, Japan OAK
MATSUMOTO, Kotaro; TAKEUCHI, Toshihiko; YOKOYAMA, Naoaki; KATAGIRI, Yoshito; OOSHIRO, Mamoru; ZAKIMI, Satoshi; Gaowa; KAWAMORI, Fumihiko; OHASHI, Norio; INOKUMA, Hisashi.
We collected a total of 206 Haemaphysalis longicornis ticks by flagging in pastures in Yonaguni Island, Okinawa, Japan, in April 2008. Four of the 206 tick DNA samples tested were positive in a polymerase chain reaction (PCR) screening for the 16SrRNA gene of Anaplasmataceae. Partial sequences of 4 PCR products were identical to each other. Longer sequences of the 16SrRNA gene were successfully determined in 2 of the 4 tick samples, and the obtained 1,392 bp and 1,300 bp sequences revealed high similarity to the 16SrRNA gene sequences of the validated Ehrlichia species, including Ehrlichia ewingii, E. chaffeensis, and E. canis (98.3–98.6%). We also sequenced 1,304 bp of the groEL gene from the 2 tick samples, and found that these had the highest similarity...
Palavras-chave: 16SrRNA; Ehrlichia; GroEL; Haemaphysalis longicornis.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3581
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Optic pathway degeneration in Japanese black cattle OAK
CHIBA, Shiori; FUNATO, Shingo; HORIUCHI, Noriyuki; MATSUMOTO, Kotaro; INOKUMA, Hisashi; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 松本, 高太郎; 猪熊, 壽; 古岡, 秀文; 古林, 与志安.
Degeneration of the optic pathway has been reported in various animal species including cattle. We experienced a case of bilateral optic tract degeneration characterized by severe gliosis in a Japanese black cattle without any obvious visual defects. To evaluate the significance, pathological nature and pathogenesis of the lesions, we examined the optic pathway in 60 cattle (41 Japanese black, 13 Holstein and 6 crossbreed) with or without ocular abnormalities. None of these animals had optic canal stenosis. Degenerative changes with severe gliosis in the optic pathway, which includes the optic nerve, optic chiasm and optic tract, were only observed in 8 Japanese black cattle with or without ocular abnormalities. Furthermore, strong immunoreactivity of...
Palavras-chave: Axonal degeneration; Gliosis; Japanese black cattle; Optic pathway degeneration.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4123
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Dual Presence of Anaplasma phagocytophilum and Its Closely Related Anaplasma sp. in Ixodid Ticks in Hokkaido, Japan, and Their Specific Molecular Detection OAK
YBAÑEZ, Adrian Patalinghug; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
Anaplasma phagocytophilum causes human granulocytic anaplasmosis (HGA) and tick-borne fever in ruminants. A closely related and potentially novel Anaplasma sp. in Japan was recently characterized. The aims of the study were to provide molecular evidence for the presence of these 2 species in Japan, and to develop a reliable PCR method based on the nucleotide differences within the citrate synthase (gltA) gene. DNA samples from 182 ixodid ticks (134 Ixodes persulcatus, 35 Haemaphysalis douglasii and 13 I. ovatus) collected from 2 sites in Hokkaido, Japan, were screened for A. phagocytophilum and its closely related Anaplasma sp. (herein designated as Anaplasma sp. Japan) using 16S rRNA PCR, revealing a combined prevalence rate of 27.5% (50 samples). The...
Palavras-chave: 16S rRNA; Anaplasma phagocytophilum; Anaplasma sp.; GltA; GroEL.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3839
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ポータブルX線撮影装置を用いた牛の Computed Radiography 画像化因子が画質に与える影響 OAK
岸本, 海織; 住谷, 峻; 李, 奇子; 清水, 純一郎; 山川, 和宏; 滄木, 孝弘; 羽田, 真悟; 松本, 高太郎; 石井, 三都夫; 佐々木, 直樹; 猪熊, 壽; 山田, 一孝; KISHIMOTO, Miori; SUMIYA, Takashi; LEE, Ki-Ja; SHIMIZU, Junichiro; YAMAKAWA, Kazuhiro; AOKI, Takahiro; HANEDA, Shingo; MATSUMOTO, Kotaro; ISHII, Mitsuo; SASAKI, Naoki; INOKUMA, Hisashi; YAMADA, Kazutaka.
牛臨床におけるComputed Radiography (CR) の有用性を確立するため,管球-カセッテ間距離およびImaging Plate (IP) の読み取り時間が画質に与える影響について検討した.管球-カセッテ間距離は画質の視覚的スコア(粒状性,鮮鋭性)に影響しなかった. IPの読み取り時間は画質の鮮鋭性と相関しなかったが,粒状性と相関が認められた.しかし読み取り時聞が12時間以内の場合には問題にならない程度であると考えられた.管球-力セッテ間距離を再現よく保つことが難しく, IP読み取り時間のばらつきが想定される牛の臨床画像診断においてCRは有用なX線撮影システムであると考えられた. The purpose of this study was to investigate the effect of X-ray tube-cassette distance and image scanning time on image quality to establish the benefit of Computed Radiography (CR) in bovine clinical practice. The tube-cassette distance had no effect on the visual evaluation score (graininess and sharpness). The image scanning time correlated with graininess but not sharpness. From these results,it was concluded that accidental variations in the tube-cassette...
Palavras-chave: ; Computed Radiography (CR); フェーデイング; Imaging Plate (IP); ポータブルX線発生装置; Bovine; Computed Radiography (CR); Fading; Imaging Plate (lP); Portable x-ray generator.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2939
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慢性子宮内膜炎を呈したホルスタイン種雌牛にみられた牛白血病の1症例 OAK
竹内, 俊彦; 吉本, 薫; 駒形, 真; 福中, 守人; 古林, 与志安; 松本, 高太郎; 猪熊, 壽; TAKEUCHI, Toshihiko; YOSHIMOTO, Kaoru; KOMAGATA, Makoto; FUKUNAKA, Morito; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; INOKUMA, Hisashi.
5歳5カ月齢のホルスタイン種雌牛が,発熱,食欲不振及び胎盤停滞を呈した.悪露排出及び子宮の腫脹は治療で改善がみられなかった.体表リンパ節の腫大はみられなかったが,直腸検査で子宮の硬結を認めたため,牛白血病を疑った.病理解剖では腹腔内リンパ節の腫大,胃漿膜面の腫瘤形成及び子宮壁の肥厚が認められた.また,組織所見ではこれら組織へのリンパ球様腫瘍細胞の浸潤が認められた.腫瘍細胞はB細胞マーカー陽性であった.牛白血病ウイルス(BLV)抗体は陰性であったが,末梢血のBLVプロウイルス検査では陽性が確認され,本症例におけるBLVの関与が示唆された. A 5-year-5-month old Holstein cow was presented with fever, anorexia, and retained placenta. Postnatal vaginal discharge and enlargement of the uterus did not improve after treatment. Although the superficial lymph nodes were not enlarged, enzootic bovine leukosis was suspected because of the enlarged and indurated uterus. An autopsy revealed enlargement of most abdominal lymph nodes, tumor mass formation on the omasum and abomasum, and thickening of the uterine wall. Histologically, there...
Palavras-chave: 牛白血病ウイルス; 慢性子宮内膜炎; 地方病性牛白血病; Bovine leukosis virus; Chronic endometritis; Enzootic bovine leukosis.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3555
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Prevalence and Risk Factor Analysis of Bovine Hemoplasma Infection by Direct PCR in Eastern Hokkaido, Japan OAK
TAGAWA, Michihito; YBANEZ, Adrian P.; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
The prevalence and risk factors of 2 bovine hemoplasma species—Mycoplasma wenyonii (Mw) and “Candidatus Mycoplasma haemobos” (CMh)—were examined by direct PCR using whole blood samples collected from 343 cattle in Eastern Hokkaido, Japan. The sensitivity of the direct PCR assay was 10-fold higher than the standard PCR assay, detecting 5 copies of bovine hemoplasma DNA per reaction. An epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was performed to assess infection risk factors. The prevalence rates were 38.5% for Mw and 39.1% for CMh, respectively, with an overall prevalence rate of 64.7% for all bovine hemoplasma infections. Significant differences were found in sampling location, living conditions...
Palavras-chave: Bovine hemoplasma; Direct PCR; Japan; Risk factor.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3838
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脳幹部膿瘍により神経症状を呈したホルスタイン種子牛の1 例 OAK
松本, 高太郎; 村上, 智亮; 菅生, 樹春; 山田, 一孝; 古林, 与志安; 松井, 高峯; 猪熊, 壽; MATSUMOTO, Kotaro; MURAKAMI, Tomotaka; SUGO, Kiharu; YAMADA, Kazutaka; KOBAYASHI, Yoshiyasu; MATSUI, Takane; INOKUMA, Hisashi.
17日齢のホルスタイン種雌子牛が,神経症状を示した. 病畜は頭部を左後方に折り曲げて伏臥姿勢を示し,時折自力で起立したがすぐに倒れ歩行不能であった. 神経学的検査では右の対光反射,威嚇瞬き反射,眼瞼反射, および音響耳介反射が消失していた. ビタミンB1,およびデキサメサゾンの投与により第7病日に歩行可能となったものの左への斜頸・旋回が認められ,神経学的検査所見に改善は認められなかった. 第7病日に病理解剖を行い,肉眼的には橋の断面右側に直径1cm大の膿瘍を認め,嫌気培養にてFusobacterium necrophorumが検出された. 左側への斜頸,聴覚の消失等の脳神経疾状は,橋の右側の膿瘍により内耳神経等の脳神経核が圧迫された結果出現した聴覚障害,平衡障害のためと考えられた. A 17-day-0ld female Holstein calf exhibited the following neurological symptoms: the calf bent its neck to the left,turning its face backward and could stand alone,but would fall within a short time. Neurological examination further revealed losses of pupillary light reflex, menace response,palpebral reflex,and hearing on the right side. After administration of dexamethasone and vitamin B1,the calf was able to walk...
Palavras-chave: 脳幹部膿瘍; ; Fusobacterium necrophorum; Brain stem abscess; Cattle.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2945
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Effect of Chronic Hemoplasma Infection on Cattle Productivity OAK
TAGAWA, Michihito; YAMAKAWA, Kazuhiro; AOKI, Takahiro; MATSUMOTO, Kotaro; ISHII, Mitsuo; INOKUMA, Hisashi; 滄木, 孝弘; 松本, 高太郎; 石井, 三都夫; 猪熊, 壽.
The present study evaluated the effect of hemoplasmosis on cattle productivity. Prevalence of bovine hemoplasma was examined by polymerase chain reaction (PCR) using whole blood samples collected from 93 breeding cows and their 71 calves in Hokkaido, Japan. Monthly milk production records and other clinical data were compared between Mycoplasma wenyonii (Mw)-infected, “Candidatus Mycoplasma haemobos” (CMh)-infected, co-infected and PCR-negative groups. Blood chemical parameters were obtained from the 93 cows and 64 calves. PCR results showed that 89.2% (83/93) of cows and 14.1% (10/71) of calves were positive for bovine hemoplasma. Based on productivity data obtained from the 93 cows, Mw-infected, CMh-infected and co-infected cows had significantly lower...
Palavras-chave: Cattle; Direct PCR; Hemoplasma; Productivity.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3870
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Molecular Epidemiological Survey of Theileria orientalis in Thua Thien Hue Province, Vietnam OAK
KHUKHUU, Altangerel; LAN, Dinh Thi Bich; LONG, Phung Thang; UENO, Akio; LI, Yan; LUO, Yuzi; MACEDO, Alan Caine Costa de; MATSUMOTO, Kotaro; INOKUMA, Hisashi; KAWAZU, Shin-Ichiro; IGARASHI, Ikuo; XUAN, Xuenan; YOKOYAMA, Naoaki; 松本, 高太郎; 猪熊, 壽; 河津, 信一郎; 五十嵐, 郁男; 玄, 学南; 横山, 直明.
Theileria orientalis is a benign bovine protozoan parasite that occasionally causes serious economic loss in the livestock industry. We report the findings of a molecular epidemiological survey of T. orientalis in 94 Vietnamese yellow cattle, 43 water buffaloes, 21 sheep, 21 goats and 85 blood-sucking ticks of cattle in the Thua Thien Hue province of Vietnam. The major piroplasm surface protein (MPSP) gene of T. orientalis was detected using polymerase chain reaction from 13 cattle (13.8%), 11 water buffaloes (25.6%), 1 sheep (4.8%) and 9 ticks (10.6%). Phylogenetic analysis using MPSP gene sequences showed the presence of seven genotypes, four previously categorized genotypes (Types 1, 3, 5 and 7) and three new genotypes (Types N-1, N-2 and N-3).
Palavras-chave: Cattle; Genotype; MPSP; Theileria orientalis; Vietnam.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3585
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乳牛の血様液貯留を特徴とする特発性心嚢水腫症例と心膜炎症例の臨床病理学的検査所見の比較 OAK
猪熊, 壽; 滄木, 孝弘; 山川, 和宏; 竹内, 俊彦; 松本, 高太郎; 石井, 三都夫; 古林, 与志安; 古岡, 秀文; INOKUMA, Hisashi; AOKI, Takahiro; YAMAKAWA, Kazuhiro; TAKEUCHI, Toshihiko; MATSUMOTO, Kotaro; ISHII, Mitsuo; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi.
病理学的に血様液貯留を特徴とする特発性心嚢水腫と診断された乳牛8症例(血様心嚢水貯留群)の臨床病理学的検査所見を回顧的に取りまとめ,心膜炎7症例(心膜炎群)の所見と比較した.血様心嚢水貯留群では冷性浮腫を呈する症例が心膜炎群に比べて多かったが,その他の臨床症状には両群で差がなかった.心エコー検査では血様心嚢水貯留群では,心膜炎でみられる心嚢内の顕著なフィブリン析出はなかった.血液及び生化学検査では,血様心嚢水貯留群では炎症像が弱く,心膜炎群と比較して白血球数,分葉核好中球割合,A/G比,アルブミン濃度,γグロブリン割合に有意差がみられた.両群でALP及びGGTの高値がみられたが,特に血様心嚢水貯留群のGGT増加は顕著であった.血様心嚢水貯留症例の診断は臨床症状だけでは困難であるが,心エコー検査所見,弱い炎症像,ALP及びGGTの高値などが心膜炎との鑑別診断に有用と考えられた. Clinicopathological findings of eight cases of idiopathic hydropericardium characterized by bloodhlike fluid (HBFhgroup) that were confirmed by necropsy were retrospectively summarized and compared with those of seven cows with pericarditis. Although the numbers of cows that showed edema in the HBFhgroup were larger than those in the pericarditishgroup, the frequency of...
Palavras-chave: 特発性心餒水腫; 心膜炎; 血様心嚢水; ; Idiopathic hydropericardium; Pericarditis; Blood-like pericardium; Cow.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3557
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Molecular Diagnosis of Infective Endocarditis Caused by Streptococcus canis in a Dog OAK
田川, 道人; 大橋, 英二; 本村, 絹代; 千葉, 史織; 堀内, 雅之; 三好, 雅史; 猪熊, 壽; 松本, 高太郎; TAGAWA, Michihito; OHASHI, Eiji; MOTOMURA, Kinuyo; CHIBA, Shiori; HORIUCHI, Noriyuki; MIYOSHI, Masahumi; INOKUMA, Hisashi; MATSUMOTO, Kotaro.
10歳の雄の雑種犬が,3週間前から持続する発熱及び元気消失を主訴に帯広畜産大学動物医療センターを受診した.心雑音及び心エコー検査において僧帽弁の疣贅物が確認され,抗生物質等により加療したが,第7病日より心不全の悪化を認め,第10病日に肺水腫により斃死した.病理解剖では,軽度の心拡大及び僧帽弁における多中心性疣贅物が認められた.血液培養検査では,Staphylococcus haemolyticusが検出されたが,疣贅物を用いたPCR検査ではStreptococcus canisと100%一致する塩基配列が得られた.血液培養分離菌はコンタミネーションであった可能性が高く,本症例の心内膜炎はStreptococcus canisによるものと考えられた.血液培養結果と実際の心内膜炎起因菌が異なる場合があることを考慮する必要がある.
Palavras-chave: 心内膜炎; PCR; Streptococcus canis.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4232
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Prevalence and Molecular Analyses of Hemotrophic Mycoplasma spp. (Hemoplasmas) Detected in Sika Deer (Cervus nippon yesoensis) in Japan OAK
TAGAWA, Michihito; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
Hemotropic mycoplasmas (hemoplasmas) are cell-wall deficient, epierythrocytic bacteria that cause infectious anemia in several mammalian species. The prevalence of hemoplasma species was examined by screening and species-specific PCR using blood samples collected from 51 sika deer in Hokkaido, Japan. Molecular analyses were performed for the 16S rRNA, 23S rRNA and RNase P RNA (rnpB) gene sequences. A total of 23/51 (45%) deer DNA samples were positive for hemoplasmas in the screening PCR. Using species-specific PCR, 12 and 17 samples were positive for ‘Candidatus Mycoplasma haemocervae’ and ‘Candidatus M. erythrocervae’, respectively. Sequencing and phylogenetic trees of those three genes indicate that the ‘Candidatus M. haemocervae’ and ‘Candidatus M....
Palavras-chave: Cervus nippon yesoensis; Hemoplasma; Japan; Phylogeny.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4012
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