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	Provedor de dados BJMBR (2) Autor Macedo,D.V. (2) Alves,A.A. (1) Brenzikofer,R. (1) Dabbeni-Sala,F. (1) Machado,E.F.A. (1) Molnar,A.M. (1) Normand,A.C.R. (1) Nunes,L.A.S. (1) Pereira-da-Silva,L. (1) Mais... Palavra-chave BN-PAGE (1) Colorimetric staining (1) Exhaustive physical exercise (1) General anesthetic (1) Glycogenolysis (1) Hemolysis (1) Ketamine (1) Mitochondrial complexes (1) Rats (1) Zoletil (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2009 (1) 2004 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Evaluation by blue native polyacrylamide electrophoresis colorimetric staining of the effects of physical exercise on the activities of mitochondrial complexes in rat muscle BJMBR Molnar,A.M.; Alves,A.A.; Pereira-da-Silva,L.; Macedo,D.V.; Dabbeni-Sala,F.. Blue native polyacrylamide electrophoresis (BN-PAGE) is a technique developed for the analysis of membrane complexes. Combined with histochemical staining, it permits the analysis and quantification of the activities of mitochondrial oxidative phosphorylation enzymes using whole muscle homogenates, without the need to isolate muscle mitochondria. Mitochondrial complex activities were measured by emerging gels in a solution containing all specific substrates for NADH dehydrogenase and cytochrome c oxidase enzymes (complexes I and IV, respectively) and the colored bands obtained were measured by optique densitometry. The objective of the present study was the application of BN-PAGE colorimetric staining for enzymatic characterization of mitochondrial... Tipo: Info:eu-repo/semantics/article Palavras-chave: BN-PAGE; Colorimetric staining; Exhaustive physical exercise; Mitochondrial complexes. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700001 Effects of different general anesthetics on serum hemolysis and hepatic and muscular glycogenolysis in rats BJMBR Machado,E.F.A.; Normand,A.C.R.; Nunes,L.A.S.; Brenzikofer,R.; Macedo,D.V.. Anesthetics can affect the structure and biological function of tissues and systems differentially. The aim of the present study was to compare three injectable anesthetics generally used in experiments with animals in terms of the degree of hemolysis and glycogenolysis occurring after profound anesthesia. Twenty-four male Wistar rats (330-440 g) were divided into three groups (N = 8): chloral hydrate (CH), ketamine + xylazine (KX), Zoletil 50® (zolazepam and tiletamine) + xylazine (ZTX). After deep anesthesia, total blood was collected. The liver and white (WG) and red gastrocnemius (RG) muscles were also immediately removed. The degree of serum hemolysis was quantified on the basis of hemoglobin concentration (g/L). Hepatic and muscular glycogen... Tipo: Info:eu-repo/semantics/other Palavras-chave: General anesthetic; Rats; Glycogenolysis; Hemolysis; Zoletil; Ketamine. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001100006 Registros recuperados: 2 Primeira ... 1 ... Última

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