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	Provedor de dados BJM (4) Ciênc. Tecnol. Aliment. (1) Ciência e Agrotecnologia (1) Autor Macedo,Gabriela Alves (6) Battestin,Vania (2) Fraga,Laira Priscila (2) Pio,Tatiana Fontes (2) Fleuri,Luciana Francisco (1) Lopes,Danielle Branta (1) Macedo,Juliana Alves (1) Matsuda,Luis Katsumi (1) Schons,Patrícia Fernanda (1) Suzuki,Gaby Tiemi (1) Mais... Palavra-chave Cutinase (3) Esterase (2) Biotin (1) Candida sp (1) Esterase specific activity (1) Fermentation (1) Fermentation process (1) Fungos (1) Fusarium oxysporum (1) Inoculum (1) Lipase (1) Lipase specific activity (1) Macroscopic characteristics (1) Media development (1) Microbial enzymes (1) Phytase (1) Resíduos (1) Riboflavin (1) Screening (1) Sorghum (1) Mais... Tipo do documento Info:eu-repo/semantics/article (6) Ano 2012 (1) 2011 (2) 2008 (1) 2005 (2) País Brazil (6) Idioma Inglês (5) Português (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Inoculum padronization for the production of cutinase by Fusarium oxysporum BJM Pio,Tatiana Fontes; Fraga,Laira Priscila; Macedo,Gabriela Alves. Cutinase is a versatile enzyme showing several interesting properties for application in industrial processes. The widespread use of this enzyme depends on the development of an efficient and low-cost production system. One of the most important steps in a fermentation process is the standardization of the inoculum characteristics. In this study, the production of cutinase by Fusarium oxysporum showed a statistically significant relationship with both the inoculum size and the inoculum PDA pH. The greatest activities were 19.1 U/mL at PDA pH 7.0 and 22.72 U/mL using an aliquot of 12.72 x 10(7) spores/mL. The macroscopic characteristics of the colonies of Fusarium oxysporum changed according to the variation of the medium pH, with the best results recorded... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium oxysporum; Inoculum; Cutinase; Macroscopic characteristics; Fermentation process. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100018 Fermentation and enzyme treatments for sorghum BJM Schons,Patrícia Fernanda; Battestin,Vania; Macedo,Gabriela Alves. Sorghum (Sorghum bicolor Moench) is the fifth most produced cereal worldwide. However, some varieties of this cereal contain antinutritional factors, such as tannins and phytate that may form stable complexes with proteins and minerals which decreases digestibility and nutritional value. The present study sought to diminish antinutritional tannins and phytate present in sorghum grains. Three different treatments were studied for that purpose, using enzymes tannase (945 U/Kg sorghum), phytase (2640 U/Kg sorghum) and Paecilomyces variotii (1.6 X 10(7) spores/mL); A) Tannase, phytase and Paecilomyces variotii, during 5 and 10 days; B) An innovative blend made of tanase and phytase for 5 days followed by a Pv increase for 5 more days; C) a third treatment... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermentation; Tannase; Phytase; Sorghum. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100010 Medium composition influence on Biotin and Riboflavin production by newly isolated Candida sp BJM Suzuki,Gaby Tiemi; Macedo,Juliana Alves; Macedo,Gabriela Alves. Complex B vitamins as Biotin and Riboflavin are required by living organisms, not only for growth but also for metabolite production, and the feed market classifies them as growth promoters. Since Brazil will soon be one of the world's biggest animal protein producers, feed production is a large consumer of vitamins and micronutrients. The industry requires 10 mg riboflavin/0.2 mg biotin per kilogram of feed; a ratio of 40 ~ 50:1. Although few studies have been conducted specifically on riboflavin production using factorial design and surface response method as an optimization strategy, it is a common practice in biotechnology with many research reports available. However, there are no reports on the use of statistical design for biotin production. This... Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida sp; Riboflavin; Biotin; Media development; Vitamin production. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300030 A rapid screening method for cutinase producing microorganisms BJM Macedo,Gabriela Alves; Pio,Tatiana Fontes. Both cutinase and lipase belong to the esterase group of enzymes (EC 3.1.1.X), which are capable of catalyzing the hydrolysis of ester bonds. Cutinase catalyzes the hydrolysis of cutin, an insoluble biopolyester which is the structural component of plant cuticles. As cutinase is an efficient catalyst in watery or organic media, it is potentially appropriate for the detergent, food and cosmetic industries. The objective of this work was to isolate microorganisms from plants and perform a pre-selection of molds showing esterase producing ability. The selected strains were then submitted to fermentation in media containing cutin. The lipolytic and cutinolytic activities of the supernatant were determined in order to differentiate lipase producers from... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutinase; Esterase; Microbial enzymes; Screening. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400016 Seleção de fungos produtores de tanase em resíduos vegetais ricos em taninos Ciência e Agrotecnologia Macedo,Gabriela Alves; Matsuda,Luis Katsumi; Battestin,Vania. Tanino acil hidrolase conhecida como tanase (E.C: 3.1.1.20) é uma enzima que hidrolisa ésteres e ligações laterais de taninos hidrolisáveis produzindo glicose e ácido gálico. A tanase é uma enzima extracelular, induzível, produzida por fungos, bactérias e leveduras pela fermentação sólida, líquida ou submersa. A fermentação sólida para a produção desta enzima oferece um grande número de vantagens sobre o método de fermentação submersa e líquida convencional. O meio de produção é simples, utiliza resíduos vegetais de uva, cajú, café ou subprodutos como farelo de trigo, arroz ou aveia, acrescidos de ácido tânico. A aplicação de resíduos é uma forma de utilizar substratos alternativos e solucionar problemas de poluição que possam causar. A tanase tem vasta... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungos; Tanase; Resíduos; Taninos. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000400016 Lipase and esterase: to what extent can this classification be applied accurately? Ciênc. Tecnol. Aliment. Lopes,Danielle Branta; Fraga,Laira Priscila; Fleuri,Luciana Francisco; Macedo,Gabriela Alves. Enzyme technology is an ever-growing field of knowledge and, in recent years, this technology has raised renewed interest, due to the search for new paradigms in several productive processes. Lipases, esterases and cutinases are enzymes used in a wide range of processes involving synthesis and hydrolysis reactions. The objective of this work was to investigate and compare the specific lipase and esterase activities of five enzymes - four already classified as lipases and one classified as cutinase - in the presence of natural and synthetic substrates. All tested enzymes presented both esterase and lipase specific activities. The highest specific esterase activity was observed for Aspergillus 1068 lipase in natural substrate and for F. oxysporum cutinase in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Lipase; Esterase; Cutinase; Lipase specific activity; Esterase specific activity. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000300009 Registros recuperados: 6 Primeira ... 1 ... Última

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