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| Registros recuperados: 18 | |
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| Reis,Marcelo S.; Takita,Marco A.; Palmieri,Darío A.; Machado,Marcos A.. |
| In this work we describe all the computational environments, pipelines, and web services developed for the CitEST transcriptome project, on which all the annotation researchers relied. We also present a complete list of CitEST libraries and, for each of them, the general features after the in silico processing, showing some quantitative information. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transcriptomics; Comparative genomics; Clustering; Trimming; Digital northern. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500031 |
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| Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Targon,Maria Luisa P.N.; Carlos,Eduardo F.; Locali-Fabris,Eliane C.; Amaral,Alexandre M.; Freitas-Astúa,Juliana; Silva-Pinhati,Ana Carla O.; Boscariol-Camargo,Raquel L.; Berger,Irving J.; Rodrigues,Carolina M.; Reis,Marcelo S.; Machado,Marcos A.. |
| In order to understand the genetic responses resulting from physiological changes that occur in plants displaying citrus variegated chlorosis (CVC) symptoms, we adopted a strategy of comparing two EST libraries from sweet orange [Citrus sinensis (L.) Osbeck]. One of them was prepared with plants showing typical CVC symptoms caused by Xylella fastidiosa and the other with non-inoculated plants. We obtained 15,944 ESTs by sequencing the two cDNA libraries. Using an in silico hybridization strategy, 37 genes were found to have significant variation at the transcriptional level. Within this subset, 21 were up-regulated and 16 were down-regulated in plants with CVC. The main functional categories of the down-regulated transcripts in plants with CVC were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sweet orange; EST; CVC; Compatible interaction. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500023 |
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| Souza,Alessandra A. de; Takita,Marco A.; Coletta-Filho,Helvécio D.; Campos,Magnólia A.; Teixeira,Juliana E.C.; Targon,Maria Luísa P.N.; Carlos,Eduardo F.; Ravasi,Juliano F.; Fischer,Carlos N.; Machado,Marcos A.. |
| The Citrus ESTs Sequencing Project (CitEST) conducted at Centro APTA Citros Sylvio Moreira/IAC has identified and catalogued ESTs representing a set of citrus genes expressed under relevant stress responses, including diseases such as citrus variegated chlorosis (CVC), caused by Xylella fastidiosa. All sweet orange (Citrus sinensis L. Osb.) varieties are susceptible to X. fastidiosa. On the other hand, mandarins (C. reticulata Blanco) are considered tolerant or resistant to the disease, although the bacterium can be sporadically detected within the trees, but no disease symptoms or economic losses are observed. To study their genetic responses to the presence of X. fastidiosa, we have compared EST libraries of leaf tissue of sweet orange Pêra IAC (highly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ESTs; Citrus variegated chlorosis; Biotic stress; CitEST; Phytopathogen. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500024 |
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| Freitas-Astúa,Juliana; Bastianel,Marinês; Locali-Fabris,Eliane C.; Novelli,Valdenice M.; Silva-Pinhati,Ana Carla; Basílio-Palmieri,Ana Carolina; Targon,Maria Luisa P.N.; Machado,Marcos A.. |
| Leprosis, caused by Citrus leprosis virus, cytoplasmic type (CiLV-C), is the main viral disease in the Brazilian citrus industry. This occurs because of the widespread source of inoculum and the year-round presence of the vector, the tenuipalpid mite Brevipalpus phoenicis, in citrus plants. In addition, while some Citrus species are resistant to CiLV-C, C. sinensis, the main cultivated species in the country, is extremely susceptible to the disease. The main objective of this work was to identify genes in C. sinensis cv. Pêra plants that were differentially expressed after the host was challenged with CiLV-C. In order to accomplish that, cDNA libraries were constructed from healthy and CiLV-inoculated sweet orange leaves. Two hundred and fifty-four genes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EST; Disease; CiLV; In silico hybridization; Sweet orange; Citrus sinensis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500026 |
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| Boscariol-Camargo,Raquel L.; Berger,Irving J.; Souza,Alessandra A.; Amaral,Alexandre M. do; Carlos,Eduardo F.; Freitas-Astúa,Juliana; Takita,Marco A.; Targon,Maria Luisa P.N.; Medina,Camilo L.; Reis,Marcelo S.; Machado,Marcos A.. |
| CitEST project resulted in the construction of cDNA libraries from different Citrus sp. tissues under various physiological conditions. Among them, plantlets of Rangpur lime were exposed to hydroponic conditions with and without water stress using PEG6000. RNA from roots was obtained and generated a total of 4,130 valid cDNA reads, with 2,020 from the non-stressed condition and 2,110 from the stressed set. Bioinformatic analyses measured the frequency of each read in the libraries and yielded an in silico transcriptional profile for each condition. A total of 40 contigs were differentially expressed and allowed to detect up-regulated homologue sequences to well known genes involved in stress response, such as aquaporins, dehydrin, sucrose synthase, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Expressed sequence tags; Transcription; Rootstock; Drought; Molecular response. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500019 |
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| Berger,Irving J.; Freitas-Astúa,Juliana; Reis,Marcelo S.; Targon,Maria Luísa P.N.; Machado,Marcos A.. |
| Out of the 18,942 flavedo expressed sequences (clusters plus singletons) in Citrus sinensis from the Citrus EST Project (CitEST), 25 were statistically supported to be differentially expressed in this tissue after a double in silico hybridization strategy against leaf-, flower-, and bark-derived ESTs. Five of them, two terpene synthases and three O-methyltransferases, are absent in the other citrus tissues with concomitant 2x2 statistics, supporting the hypothesis that they are putative flavedo-specific expressed sequences. The pattern of these differentially expressed sequences during fruit development suggests that most of them are developmentally regulated. Some expressed gene products, including a putative germin-like protein highly expressed in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EST; Fruit; Gene expression; Orange; Tissue-specific. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500004 |
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| Fietto,Luciano G.; Costa,Maximiller D.L.; Cruz,Cosme D.; Souza,Alessandra A.; Machado,Marcos A.; Fontes,Elizabeth P.B.. |
| The completion of the genome sequencing of the Arabidopsis thaliana model system provided a powerful molecular tool for comparative analysis of gene families present in the genome of economically relevant plant species. In this investigation, we used the sequences of the Arabidopsis Hsp70 gene family to identify and annotate the Citrus Hsp70 genes represented in the CitEST database. Based on sequence comparison analysis, we identified 18 clusters that were further divided into 5 subgroups encoding four mitochondrial mtHsp70s, three plastid csHsp70s, one ER luminal Hsp70 BiP, two HSP110/SSE-related proteins and eight cytosolic Hsp/Hsc70s. We also analyzed the expression profile by digital Northern of each Hsp70 transcript in different organs and in response... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chaperones; Digital northern; Heat shock proteins; Genome; Expressed sequence tags. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500017 |
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| Cristofani-Yaly,Mariângela; Berger,Irving J.; Targon,Maria Luisa P.N.; Takita,Marco A.; Dorta,Sílvia de O.; Freitas-Astúa,Juliana; Souza,Alessandra A. de; Boscariol-Camargo,Raquel L.; Reis,Marcelo S.; Machado,Marcos A.. |
| Citrus is the most important fruit crop in Brazil and Citrus tristeza virus (CTV) is considered one of the most important pathogens of citrus. Most citrus species and varieties are susceptible to CTV infection. However, Poncirus trifoliata, a close relative of citrus, is resistant to the virus. In order to better understand the responses of citrus plants to the infection of CTV, we constructed expressed sequence tag (EST) libraries with tissues collected from Poncirus trifoliata plants, inoculated or not with Citrus tristeza virus at 90 days after inoculation, grafted on Rangpur lime rootstocks. We generated 17,867 sequence tags from Poncirus trifoliata inoculated (8,926 reads) and not (8,941 reads) with a severe CTV isolate. A total of 2,782 TCs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Disease resistance; Citrus tristeza virus; Biotic stress. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500025 |
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| Campos,Magnólia A.; Rosa,Daniel D.; Teixeira,Juliana Érika C.; Targon,Maria Luisa P.N.; Souza,Alessandra A.; Paiva,Luciano V.; Stach-Machado,Dagmar R.; Machado,Marcos A.. |
| In silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Functional genome; Ests; Gene expression profiles; Pathogenesis-related proteins; Defense genes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500020 |
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| Nunes,William M.C.; Molina,Rúbia de O.; Albuquerque,Fernando A. de; Corazza-Nunes,Maria J.; Zanutto,Carlos A.; Machado,Marcos A.. |
| A Clorose Variegada dos Citros (CVC) é uma importante doença causada pela bactéria Xylella fastidiosa Wells et al., que é transmitida por insetos sugadores do xilema como vetores, denominados cigarrinhas (Hemiptera: Cicadellidae). No presente estudo, avaliou-se a flutuação populacional de espécies de cigarrinhas pertencentes às tribos Cicadellini e Proconiini, da subfamília Cicadelinae, em um pomar comercial de laranja-doce [Citrus sinensis (L.) Osb.], contendo as variedades Valência, Natal, Pêra e Folha Murcha, localizados no Noroeste do Paraná. Amostragens quinzenais foram realizadas com o uso de armadilhas adesivas amarelas, no total de 24 armadilhas em cada avaliação, de novembro de 1999 a março de 2004. As espécies mais representativas foram... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CVC; Citrus sinensis; Inseto vetor; Cicadellidae. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2007000200012 |
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| Machado,Marcos A.; Cristofani-Yaly,Mariângela; Bastianel,Marinês. |
| Although the citriculture is one of the most important economic activities in Brazil, it is based on a small number of varieties. This fact has contributed for the vulnerability of the culture regarding the phytosanitary problems. A higher number of varieties/genotypes with potential for commercial growing, either for the industry or fresh market, has been one of the main objectives of citrus breeding programs. The genetic breeding of citrus has improved, in the last decades, due to the possibility of an association between biotechnological tools and classical methods of breeding. The use of molecular markers for early selection of zygotic seedlings from controlled crosses resulted in the possibility of selection of a high number of new combination and, as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citriculture; Improvement; Hybrids. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000500019 |
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| Nickel,Osmar; Targon,Maria L.P.N.; Fajardo,Thor V.M.; Machado,Marcos A.; Trivilin,Ana P.. |
| The coat protein gene of Apple stem grooving virus (ASGV) was amplified by RT-PCR, cloned, sequenced and subcloned in the expression vector pMal-c2. This plasmid was used to transform Escherichia coli BL21c+ competent cells. The ASGV coat protein (cp) was expressed as a fusion protein containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP fusion protein was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fusion protein gave specific reactions to ASGV from infected apple (Malus domestica) cv. Fuji Irradiada and Chenopodium quinoa at dilutions of up to 1:1,000 and 1:2,000, respectively, in plate trapped ELISA. The ASGVcp/MBP... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Recombinant antigen; Latent virus; ELISA; Malus domestica. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000500017 |
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| Bellato,Cláudia de M.; Garcia,Andréia K.M.; Mestrinelli,Fabiana; Tsai,Siu M.; Machado,Marcos A.; Meinhardt,Lyndel W.. |
| An in vitro system was developed to induce and identify Xylella fastidiosa proteins that were differentially expressed in the presence of callus-derived extracts from its host, the citrus cultivar Pêra. To optimize the induction, we first developed a single culture medium for the growth of both, host and bacteria. This medium, CPXPm7, which mimics the citrus xylem sap, showed that X. fastidiosa at 72 h post-incubation had 10(8) colony forming units mL-1, while Pêra cells had the highest fresh weight content (0.79 g). After testing various methods of co-cultivation of the bacteria and host callus grown in this single medium, the best induction procedure was to grow X. fastidiosa in a solid medium amended with an extract of Pêra callus grown in CPXPm7.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2DE; Callus; Pathogenicity; Protein; Xylella-citrus interaction. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200012 |
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| Registros recuperados: 18 | |
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