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| Volotão,Aline Cardoso Caseca; Ramos,Nathália Motta Delvaux; Fantinatti,Maria; Moraes,Marcus Vinícius Proença de; Atique Netto,Halim; Storti-Melo,Luciane Moreno; Godoy,Elenir Alves Macedo de; Rossit,Andréa Regina Baptista; Fernandes,Octávio; Machado,Ricardo Luiz Dantas. |
| OBJECTIVE: In order to evaluate the potential zoonotic transmission of Giardia duodenalis, isolates from humans and dogs in the Northwestern region of the São Paulo State, Brazil were characterized based on the β-giardin gene. METHODS: The samples were analyzed by sequencing of the Nested-PCR products. RESULTS: The A1 and A2 subgenotypes were detected in human and dogs. Cysts of assemblage B, C and D have not been found in any isolates studied. CONCLUSIONS: These results are consistent with the view that giardiasis in the largest endemic region of the Brazil should not be seen as a single entity. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Giardia duodenalis; Beta giardin gene; Genotypes; Zoonosis; Brazil. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400014 |
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| Marques,Fabiana Rangel; Cardoso,Luciana Ventura; Cavasini,Carlos Eugênio; Almeida,Magali Carmem de; Bassi,Nair Aparecida; Almeida,Margarete Teresa Gottardo de; Rossit,Andréa Regina Baptista; Machado,Ricardo Luiz Dantas. |
| We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a specificity of 96%; positive and negative predictive values were 89% and 100%, respectively. The commercial ELISA and ZNm proved to be valuable diagnostic tools for Cryptosporidium infection. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Parasites; Acquired immunodeficiency syndrome; Cryptosporidium diagnosis; Brazil. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000100002 |
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