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OptiFish: Probiotika og foders betydning for sundheden hos økologisk regnbueørred Organic Eprints
Madsen, Lone.
Formålet med OPTIFISH er at sikre optimale betingelser og høj overlevelse for regnbueørreder i økologisk akvakultur. Regnbueørreden er den dominerende opdrætsfisk i dansk akvakultur, hvoraf en mindre del produceres økologisk. Pt. er der ingen produktion af økologisk yngel, da en økologisk fisk igennem livsforløbet højst må behandles med antibiotika to gange, hvilket kan være svært at overholde pga. gentagne sygdomsudbrud specielt forårsaget af bakterien Flavobacterium psychrophilum på yngelstadiet. Endvidere vides det fra lakseopdræt, at vegetabilske proteinkilder (f.eks. sojamel) i foderet påvirker tarmslimhinden, hvor der ses betændelse med en efterfølgende svækkelse af immunstatus. OPTIFISH vil se på, hvordan økologiske fodertyper med indhold af...
Tipo: Web product Palavras-chave: Animal husbandry; Feeding and growth; Health and welfare.
Ano: 2012 URL: http://orgprints.org/22265/2/22265.PDF
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Øko-foder lige så godt for regnbueørredyngel som konventionelt Organic Eprints
Madsen, Lone; Ingerslev, Hans-Christian; Dalsgaard, Inger; Boye, Mette; Buchmann, Kurt; Chettri, Jiwan K.; Jørgensen, Louise von Gersdorff; Lauritsen, Anne Hjørngaard; Henriksen, Niels Henrik.
Ingredienserne i fiskefoderet ser ud til at have større betydning end hvorvidt foderet er økologisk eller konventionelt ved opdræt af regnbueørreder. Det viser forsøg gennemført i projektet OPTIFISH.
Tipo: Newspaper or magazine article Palavras-chave: Feeding and growth; Health and welfare; Denmark.
Ano: 2014 URL: http://orgprints.org/28101/6/28101.pdf
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Foderet påvirker ørredernes tarmkanal Organic Eprints
Ingerslev, Hans-Christian; Madsen, Lone.
På DTU Veterinærinstituttet arbejdes der i øjeblikket på at karakterisere den bakterielle flora i tarmen fra yngel hos regnbueørred, som der opdrættes store mængder af i Danmark. Med en ny sekventeringsmetode - den såkaldte "næste-generations sekventering" - er det muligt at beskrive komplette sammensætninger af bakterier fra fx vævsprøver på baggrund af bakteriernes DNA-sammensætning.
Tipo: Newspaper or magazine article Palavras-chave: Feeding and growth; Health and welfare.
Ano: 2012 URL: http://orgprints.org/21752/3/21752.pdf
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The development of the gut microbiota in rainbow trout (Oncorhynchus mykiss) is affected by first feeding and diet type Organic Eprints
Ingerslev, Hans-Christian; Jørgensen, Louise von Gersdorff; Strube, M. Lenz; Larsen, N.; Dalsgaard, Inger; Boye, Mette; Madsen, Lone.
An influence of the intestinal microbiota in connection to first-feeding of rainbow trout (Oncorhynchus mykiss) fry was demonstrated using Illumina HiSeq sequencing. The period from the end of yolk-sac feeding and until seven weeks post first-feeding was examined after administration of either a marine- or plant based diet with or without the probiont Pediococcus acidilactici. Before first feeding the main part of the sequence reads grouped to the genus Sediminibacterium probably originating from the surrounding water. The microbial abundance and diversity increased after first-feeding and the microbiota then changed towards Firmicutes phylum dominance for plant based fed fish and towards dominance of phylum Proteobacteria for the marine fed fish. After...
Tipo: Journal paper Palavras-chave: Feeding and growth; Health and welfare.
Ano: 2014 URL: http://orgprints.org/27336/19/27336.pdf
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Infection models with Flavobacterium psychrophilum Organic Eprints
Madsen, Lone; Dalsgaard, Inger.
High mortalities among rainbow trout (Oncorhynchus mykiss) fry are often the result of infections caused by Flavobacterium psychrophilum, and in Denmark this pathogen can be found in nearly all freshwater fish farms. The infection can be treated with antibiotics, but lower sensitivity against the infection is an increasing problem for some of the used drugs, wherefore alternative treatment methods (e.g. bacteriophages) are investigated. Prophylactic measures (e.g. feed additives) are another approach, so infections with the bacterium can be avoided, before they become a problem. No matter if the focus is on prophylactic or alternative treatment, reliable infection models are needed to evaluate the treatments as well as to get more knowledge about...
Tipo: Conference paper, poster, etc. Palavras-chave: Production systems; Health and welfare; Aquaculture; Denmark.
Ano: 2015 URL: http://orgprints.org/30002/1/Abstract%20Madsen%20and%20Dalsgaard%20Flavobacterium%202015.pdf
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THE INTESTINAL MICROBIOTA IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) IS INFLUENCED BY DIET TYPE AND YERSINIA RUCKERI CHALLENGE Organic Eprints
Ingerslev, Hans-Christian; Dalsgaard, Inger; Jørgensen, Louise von Gersdorff; Madsen, Lone.
In recent years it has become more and more evident that the bacterial flora in the gut of warm-blooded animals modulates physiological processes and the immunological status of the host. Besides effects on growth parameters, commensal intestinal bacteria balance the immune system and prevent colonization of pathogenic bacteria. The question is if the gut microbiota is also important in lower vertebrates such as fish? Is the microbiota related to the diet type and does it play a protective role in connection to pathogenic challenge? To examine these questions rainbow trout fry were fed two different diets of either a marine or vegetable origin from first feeding and onwards. At a size of about four gram the fish were bath challenged by Yersinia ruckeri...
Tipo: Conference paper, poster, etc. Palavras-chave: Feeding and growth; Health and welfare; Denmark.
Ano: 2013 URL: http://orgprints.org/24227/9/24227.pdf
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SHELTERFISH New tools to improve fish health and environment in organic aquaculture Organic Eprints
Jokumsen, Alfred; Gesto, Manuel; Buchmann, Kurt; Lorenzen, Niels; Pedersen, Lars-Flemming; Henriksen, Niels Henrik; Schmidt, Jacob; Madsen, Lone; Kania, Per; Pedersen, Per Bovbjerg.
Rainbow trout farming and in particular organic rainbow trout farms are critically challenged by the relatively high prevalence of skin/gill infections caused by various pathogens, especially the parasite Costia (Ichthyobodo necator) and amoebae, which are ultimately lethal for fry/smaller fish. In addition, a Midichloria-like bacterium causes the non-lethal skin disease Red Mark Syndrome (RMS), which results in downgrading/rejection of up to 30% of the fish when placed on the market. Treatment by use of antibiotics/parasitics/ auxiliary compounds is only possible to a limited extent in organic trout production. Hence, solutions to prevent and/or treat costia, amoebae and RMS are urgently needed, not only to secure production of organic rainbow trout in...
Tipo: Conference paper, poster, etc. Palavras-chave: Production systems; Health and welfare; Aquaculture.
Ano: 2019 URL: http://orgprints.org/36515/1/Jokumsen_ShelterFish_Poster_.pdf
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Rapport fra RobustFish temadag d. 5. april 2018 Organic Eprints
Jokumsen, Alfred; Gesto, Manuel; Lund, Ivar; Madsen, Lone; Andersen R., Nikolaj; Pedersen, Lars-Flemming; Larsen, Erling P.; Nielsen, Max; Nielsen, Thorkild; Larsen, Villy J..
Formålet med RobustFish projektet var at: • Styrke udviklingen af økologisk ørredproduktion i Danmark men også til gavn for konventionelt opdræt • Undersøge ørredyngels robusthed i forhold til start-fodrings adfærd, fedtsyreindholdet i foderet og følsomhed over for yngeldødeligheds-syndromet (YDS) • Effekt af vandbehandling med hjælpestoffer • Viden om markedsforhold, forbrugerkendskab, produktudvikling og øget afsætning. Robustfish har adresseret ovennævnte problemstillinger, og på grundlag af opnåede resultater kan der drages følgende konklusioner og deraf følgende anbefalinger og perspektiver for styrkelse af det økologiske opdræt af regnbueørred i Danmark. • Yngel med tidlig fødesøgnings adfærd (swim-up) viste tendens til større robusthed...
Tipo: Report Palavras-chave: Production systems; Feeding and growth; Markets and trade; Health and welfare; Aquaculture.
Ano: 2018 URL: http://orgprints.org/33577/1/Workshop_Report_Presentations_FINAL.pdf
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Organic rainbow trout – does the diet composition influence the fish health? Organic Eprints
Ingerslev, Hans-Christian; Dalsgaard, Inger; Boye, Mette; Madsen, Lone.
Rainbow trout (Oncorhynchus mykiss) is the dominant fish species in Danish aquaculture and the production is yearly about 40.000 tons. Freshwater accounts for about 75% of the production, while the remaining comes from seawater. During the last few years an organic fish production has been initiated and the rates of farmed organic fish are continuously increasing. Antibiotic treatment is only allowed a limited number of times during the entire life of an organic fish and if more treatments are needed the fish must be sold with the status of being conventionally raised. Thus it is a challenge to prevent disease outbreaks, e.g. by feeding with diets providing optimal health for the fish by strengthening their immune system or the composition of the gut...
Tipo: Newspaper or magazine article Palavras-chave: Feeding and growth; Health and welfare.
Ano: 2014 URL: http://orgprints.org/27337/6/27337.pdf
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Organic fishfarming requires robust fish Organic Eprints
Andersen, G., Nikolaj; Madsen, Lone.
Da medicinbehandling af økologisk ørred er uønsket og kun er tilladt i stærkt begrænset omfang, er ynglens robusthed overfor bl.a. den alvorligste yngelsygdom i dansk ørredopdræt, YDS (”yngeldødeligheds-syndrom”), særdeles vigtig. Ynglens robusthed måles i forhold til ynglens swim-up adfærd (first feeding) og performance under opvæksten. Indholdet af specifikke essentielle Ω-3 fedtsyrer i foderet til ynglen er særlig vigtig for fiskens vækst, sundhed og velfærd, herunder robusthed overfor stress. Dette undersøges ved stress tests samt challenge tests over for YDS.
Tipo: Newspaper or magazine article Palavras-chave: Health and welfare.
Ano: 2016 URL: http://orgprints.org/30655/1/Gedsted%20Andersen_Madsen_%C3%98kologisk%20fiskeopdr%C3%A6t%20kr%C3%A6ver%20robuste%20fisk_Oct2016.pdf
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DIET TYPE AND CHALLENGE BY YERSINIA RUCKERI INFLUENCE THE INTESTINAL MICROBIOTA IN RAINBOW TROUT (ONCORHYNCHUS MYKISS) Organic Eprints
Ingerslev, Hans-Christian; Dalsgaard, Inger; Jørgensen, Louise von Gersdorff; Madsen, Lone.
In warm-blooded animals such as humans and pigs the intestinal microbial composition is dependent on the type of ingested diet. It is known that it also influences the immune system and prevent colonization of pathogenic bacteria. The question is if the gut microbiota has the same impact in lower vertebrates such as fish? To examine this rainbow trout fry were fed two different diets of either a marine or vegetable origin. At a size of about four gram a subset of the fish was bath challenged by Yersinia ruckeri serotype O1 and intestines were then sampled 5 days post challenge from challenged fish (n = 40) and non-challenged control fish (n = 40). Subsequent metagenomic examination based on the 16S rDNA gene was then performed using the Illumina HiSeq...
Tipo: Conference paper, poster, etc. Palavras-chave: Feeding and growth; Health and welfare; Denmark.
Ano: 2013 URL: http://orgprints.org/24228/3/24228.pdf
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Optimized infection models with Flavobacterium psychrophilum Organic Eprints
Madsen, Lone.
Conclusions regarding infection models with Flavobacterium psychrophilum are given. Intraperioneal injection challenge with this bacterium is highly reproducible, whereas the outcome of a bath infection challenge is depending on the stressor used in connection with the bath challenge. In general both mortalities as well as level of reproducibility is much lower compared to the intraperitoneal injection challenge.
Tipo: Other Palavras-chave: Health and welfare.
Ano: 2015 URL: http://orgprints.org/30017/1/Optimized%20infection%20models%20with%20Flavobacterium%20psychrophilum.docx
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Characterisation of the gut microflora in rainbow trout fry (Oncorhynchus mykiss) using deep-sequencing Organic Eprints
Ingerslev, Hans-Christian; Dalsgaard, Inger; Boye, Mette; Madsen, Lone.
Abstract from a DAFINET workshop: For many years it has been known that the bacterial microflora in the gut of warm-blooded animals live in harmony with the host and exert various beneficial effects on the health by their metabolic activities. Hence, the gut microbiota has a high importance for the animal. In many studies from e.g. humans and the pig mapping of the bacterial flora from the gut have shown dominance by some specific bacterial groups, and this bacterial profile is termed as a ‘core microbiota’. For lower vertebrates like fish mapping of the bacterial flora in the gastrointestinal system is to date a relatively new research field and previous studies have mainly been done on bacterial species that can be cultured or by classical molecular...
Tipo: Conference paper, poster, etc. Palavras-chave: Feeding and growth; Health and welfare.
Ano: 2012 URL: http://orgprints.org/21751/3/21751.pdf
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Økologisk regnbueørred - hvordan påvirker fodersammensætningen fiskenes sundhed? Organic Eprints
Ingerslev, Hans-Christian; Dalsgaard, Inger; Boye, Mette; Madsen, Lone.
Regnbueørred er den dominerende produktionsfisk i Dansk akvakultur, og der produceres årligt ca. 30.000 tons i ferskvandsopdræt. I de senere år er der igangsat produktion af økologiske fisk og mængden er stigende. I produktionen af økologiske regnbueørreder i akvakultur er der en øvre begrænsning på to behandlinger med antibiotika i tilfælde af sygdomsudbrud – hvis flere er nødvendige, kan fisken ikke længere sælges som værende økologisk. Derfor er der udfordringer i at undgå, at fiskene bliver syge, bl.a. ved at sørge for, at de får optimalt foder, som styrker deres immunforsvar og dermed evne til at modstå sygdom. Dette undersøges nu nørmere i Organic RDD projektet, OPTIFISH.
Tipo: Newspaper or magazine article Palavras-chave: Feeding and growth; Health and welfare.
Ano: 2012 URL: http://orgprints.org/22094/7/22094.pdf
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Stress and disease resilience differences related to emergence time for first feeding in farmed rainbow trout (Oncorhynchus mykiss) Organic Eprints
Gesto, Manuel; Madsen, Lone; Andersen, Nikolaj R.; Jokumsen, Alfred.
Salmonid individuals show a relatively high variability in the time required to abandon the gravel nest where they hatch, the so-called "emergence time". Different behavioral and physiological traits have been shown to be associated to that emergence time in wild salmonids. In general, early- and late-emerging fish have traits resembling those of proactive and reactive stress coping styles, respectively. Proactive fish are considered to be more resilient to stress and probably to disease, so it was hypothesized that fish with different emergence time have different ability to resist repeated episodes of stress without suffering deleterious effects on their welfare or health status. In this study, rainbow trout eyed eggs were hatched and larvae were...
Tipo: Journal paper Palavras-chave: Health and welfare.
Ano: 2018 URL: http://orgprints.org/32863/1/Gesto%20et%20al_Stress%20and%20disease%20resilience%20differences%20related%20to%20emergence%20time%20for%20first%20feeding%20in%201%20farmed%20rainbow%20trout%20%28Oncorhynchus%20mykiss%29manuscript_JEB_2018.pdf
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The effect of dietary fatty acid content on rainbow trout fry robustness towards Flavobacterium psychrophilum Organic Eprints
Andersen, G., Nikolaj; Lund, Ivar; Jokumsen, Alfred; Madsen, Lone.
Among the most serious rainbow trout (Oncorhynchus mykiss) fry diseases in Danish aquaculture is Rainbow Trout Fry syndrome (RTFS), caused by the bacterium Flavobacterium psychrophilum. The robustness of the rainbow trout fry towards F. psychrophilum may be related to dietary lipid composition and the content of highly unsaturated omega-3 fatty acids (HUFAs). In the project RobustFish we tested the immune competence of 1g rainbow trout fry towards F. psychrophilum by experimental challenges with the bacterium, either intraperitoneal injection (triplicate aquaria with 30 fish per aquarium) or bath infection (duplicate aquaria with 60 fish per aquarium). Additionally, four experimental control aquaria for each challenge method. From first feeding and...
Tipo: Conference paper, poster, etc. Palavras-chave: Feeding and growth; Health and welfare.
Ano: 2017 URL: http://orgprints.org/32855/1/EAFP%202017%20Abstract%20N%20Andersen%20et%20al%20017-O.pdf
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Diet type dictates the gut microbiota and the immune response against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss) Organic Eprints
Ingerslev, Hans-Christian; Strube, M. Lenz; Jørgensen, Louise von Gersdorff; Dalsgaard, Inger; Boye, Mette; Madsen, Lone.
This study investigated the influence of the rainbow trout (Oncorhynchus mykiss) commensal intestinal microbiota in connection to an experimental Yersina ruckeri infection, the causative agent of enteric redmouth disease. One marine and one plant diet was administered to two different groups of rainbow trout. The plant-based diet gave rise to an intestinal microbiota dominated by the genera Streptococcus, Leuconostoc and Weissella from phylum Firmicutes whereas phylum Proteobacteria/Bacteroidetes/Actinobacteria dominated the community in the marine fed fish. In connection to the Y. ruckeri bath challenge there was no effect of the diet type on the cumulative survival, but the number of Y. ruckeri positive fish as measured by plate count and the number of...
Tipo: Journal paper Palavras-chave: Feeding and growth; Health and welfare.
Ano: 2014 URL: http://orgprints.org/27356/7/27356.pdf
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Rainbow trout fed diets with varying content of marine and plant origin; how does that influence the outcome of experimental infections of the fry with Flavobacterium psychrophilum and Yersinia ruckeri? Organic Eprints
Madsen, Lone; Ingerslev, Hans-Christian; Boye, Mette; Dalsgaard, Inger.
Feed for rainbow trout aquaculture has traditionally been based on marine resources such as fish meal and fish oil. Because of a shortage of marine resources as well as the growing production of farmed fish, the feed industry has been forced to partially exchange fish meal protein with proteins derived from plants, like soy bean meal. This has been shown to affect the salmonid intestinal mucosa, and in addition, plant-based dietary proteins have been associated with changes in disease susceptibility in salmon and it has been suggested that these special diet types weakens the immune status of the fish. One major cause for losses in Danish freshwater fish farms is the fry disease rainbow trout fry syndrome (RTFS), caused by the bacterium Flavobacterium...
Tipo: Conference paper, poster, etc. Palavras-chave: Feeding and growth; Health and welfare.
Ano: 2014 URL: http://orgprints.org/27338/6/27338.pdf
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Yersinia ruckeri challenge on rainbow trout fed different diet types Organic Eprints
Madsen, Lone; Dalsgaard, Inger.
A challenge for sustainable rainbow trout production is an increasing use of plant sources in fish feed due to shortage of marine protein and oil sources. Diets with high plant content are known to cause enteritis and injury to the intestine, which will affect the absorption of nutrients, affecting the overall health status and welfare of the fish. The result is a higher risk of disease following exposure to pathogenic microorganisms. The aim of this study was to elucidate how different feed types with varying amounts of marine versus organic plant protein and oil sources affected the survival of rainbow trout in connection with an infection. Enteric redmouth disease caused by Yersinia ruckeri is an economically important disease which causes problems in...
Tipo: Conference paper, poster, etc. Palavras-chave: Health and welfare; Farm nutrient management.
Ano: 2010 URL: http://orgprints.org/18314/1/18314.pdf
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Studying the gut microbiota using rainbow trout as a model: the dynamics of the microbiota changes after first feeding Organic Eprints
Ingerslev, Hans-Christian; Boye, Mette; Dalsgaard, Inger; Madsen, Lone.
Poster abstract from ISME14: Fish provide an excellent model for studying the gut microbiota. During the very first life stage of most fish species nutrition only comes from a yolk sac originating from the egg and not from external feed sources. During this larval phase the intestine is being formed and becomes functional as the amount of yolk sac protein is used up and feeding from external sources slowly takes over. Thus, studying the intestine in this developmental window is useful for examining the influence of feeding on the gut microbiota dynamics. In this study we examined this in the commercially produced species rainbow trout (Oncorhynchus mykiss) using next-generation sequencing. The examined fish were reared in re-circulated freshwater...
Tipo: Conference paper, poster, etc. Palavras-chave: Feeding and growth; Health and welfare.
Ano: 2012 URL: http://orgprints.org/21753/3/21753.pdf
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