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From population connectivity to the art of striping Russian dolls: the lessons from Pocillopora corals ArchiMer
Gelin, Pauline; Fauvelot, Cecile; Bigot, Lionel; Baly, Joseph; Magalon, Helene.
Here, we examined the genetic variability in the coral genus Pocillopora, in particular within the Primary Species Hypothesis PSH09, identified by Gélin, Postaire, Fauvelot and Magalon (2017) using species delimitation methods [also named Pocillopora eydouxi/meandrina complex sensu, Schmidt-Roach, Miller, Lundgren, & Andreakis (2014)] and which was found to split into three secondary species hypotheses (SSH09a, SSH09b, and SSH09c) according to assignment tests using multi-locus genotypes (13 microsatellites). From a large sampling (2,507 colonies) achieved in three marine provinces [Western Indian Ocean (WIO), Tropical Southwestern Pacific (TSP), and Southeast Polynesia (SEP)], genetic structuring analysis conducted with two clustering analyses...
Tipo: Text Palavras-chave: Bayesian assignments; Cluster; DAPC; Microsatellites; Pocillopora; Scleractinian.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00416/52776/53649.pdf
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Isolation and characterization of eight microsatellite loci from Galeocerdo cuvier (tiger shark) and cross-amplification in Carcharhinus leucas, Carcharhinus brevipinna, Carcharhinus plumbeus and Sphyrna lewini ArchiMer
Pirog, Agathe; Jaquemet, Sebastien; Blaison, Antonin; Soria, Marc; Magalon, Helene.
The tiger shark Galeocerdo cuvier (Carcharhinidae) is a large elasmobranch suspected to have, as other apex predators, a keystone function in marine ecosystems and is currently considered Near Threatened (Red list IUCN). Knowledge on its ecology, which is crucial to design proper conservation and management plans, is very scarce. Here we describe the isolation of eight polymorphic microsatellite loci using 454 GS-FLX Titanium pyrosequencing of enriched DNA libraries. Their characteristics were tested on a population of tiger shark (n = 101) from Reunion Island (South-Western Indian Ocean). All loci were polymorphic with a number of alleles ranging from two to eight. No null alleles were detected and no linkage disequilibrium was detected after Bonferroni...
Tipo: Text Palavras-chave: Carcharhiniform; Microsatellites; Control region; Population Genetics.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00626/73769/74405.pdf
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Population differentiation or species formation across the Indian and the Pacific Oceans? An example from the brooding marine hydrozoan Macrorhynchia phoenicea ArchiMer
Postaire, Bautisse; Gelin, Pauline; Bruggemann, J. Henrich; Pratlong, Marine; Magalon, Helene.
Assessing population connectivity is necessary to construct effective marine protected areas. This connectivity depends, among other parameters, inherently on species dispersal capacities. Isolation by distance (IBD) is one of the main modes of differentiation in marine species, above all in species presenting low dispersal abilities. This study reports the genetic structuring in the tropical hydrozoan Macrorhynchia phoenicea α (sensu Postaire et al., 2016a), a brooding species, from 30 sampling sites in the Western Indian Ocean and the Tropical Southwestern Pacific, using 15 microsatellite loci. At the local scale, genet dispersal relied on asexual propagation at short distance, which was not found at larger scales. Considering one representative per...
Tipo: Text Palavras-chave: Assignment test; Brooding species; Cryptic diversity; Hydrozoa; Microsatellites.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00399/51011/51795.pdf
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Molecular species delimitation methods and population genetics data reveal extensive lineage diversity and cryptic species in Aglaopheniidae (Hydrozoa) ArchiMer
Postaire, Bautisse; Magalon, Helene; Bourmaud, Chloe A. -f.; Bruggemann, J. Henrich.
A comprehensive inventory of global biodiversity would be greatly improved by automating methods for species delimitation. The Automatic Barcode Gap Discovery method, the Poisson tree processes algorithm and the Generalized mixed Yule-coalescent model have been proposed as means of increasing the rate of biodiversity description using single locus data. We applied these methods to explore the diversity within the Aglaopheniidae, a hydrozoan family with many species widely distributed across tropical and temperate oceans. Our analyses revealed widespread cryptic diversity in this family, almost half of the morpho-species presenting several independent evolutionary lineages, as well as support for cases of synonymy. For two common species of this family,...
Tipo: Text Palavras-chave: Automatic Barcode Gap Discovery; Generalized mixed Yule-coalescent model; Poisson tree processes; 16S; Microsatellites; Cnidarians.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00348/45907/45590.pdf
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