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Chambouvet, Aurélie; Monier, Adam; Maguire, Finlay; Itoïz, Sarah; Del Campo, Javier; Elies, Philippe; Edvardsen, Bente; Eikreim, Wenche; Richards, Thomas A.. |
The Fungi are a diverse kingdom, dominating terrestrial environments and driving important ecologies. Although fungi, and the related Opisthosporidia, interact with photosynthetic organisms on land and in freshwater as parasites, symbionts, and/or saprotrophic degraders [1, 2], such interactions in the marine environment are poorly understood [3, 4, 5, 6, 7, 8]. One newly identified uncultured marine lineage has been named novel chytrid-like-clade-1 (NCLC1) [4] or basal-clone-group-I [5, 6]. We use ribosomal RNA (rRNA) encoding gene phylogenies to demonstrate that NCLC1 is a distinct branch within the Opisthosporidia (Holomycota) [7]. Opisthosporidia are a diverse and largely uncultured group that form a sister branch to the Fungi or, alternatively, the... |
Tipo: Text |
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Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00591/70337/68391.pdf |
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Richards, Thomas A.; Leonard, Guy; Mahe, Frederic; Del Campov, Javier; Romac, Sarah; Jones, Meredith D. M.; Maguire, Finlay; Dunthorn, Micah; De Vargas, Colomban; Massana, Ramon; Chambouvet, Aurelie. |
Environmental DNA and culture-based analyses have suggested that fungi are present in low diversity and in low abundance in many marine environments, especially in the upper water column. Here, we use a dual approach involving high-throughput diversity tag sequencing from both DNA and RNA templates and fluorescent cell counts to evaluate the diversity and relative abundance of fungi across marine samples taken from six European near-shore sites. We removed very rare fungal operational taxonomic units (OTUs) selecting only OTUs recovered from multiple samples for a detailed analysis. This approach identified a set of 71 fungal 'OTU clusters' that account for 66% of all the sequences assigned to the Fungi. Phylogenetic analyses demonstrated that this... |
Tipo: Text |
Palavras-chave: 454 pyrosequencing; Chytrids; Dikarya; Sediment communities. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00374/48490/48880.pdf |
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