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| Armesto,Cecilia; Maia,Fernanda Gonçalves Martins; Abreu,Mário Sobral de; Figueira,Antonia dos Reis; Silva,Bruno Marques da; Monteiro,Fernando Pereira. |
| Blister spot (Colletotrichum gloeosporioides) is now widespread in most coffee producing states of Brazil, becoming a limiting factor for production. The lack of data relating to the reproduction of typical symptoms (light green, oily patches) leaves a gap within the pathosystem, forcing the search for new methodologies for monitoring the disease. Monitoring of genetically modified organisms has proven to be an effective tool in understanding the host x pathogen interactions. Thus, the present study was carried out to evaluate the effectiveness of two systems of genetic transformation in obtaining mutants using the gfp reporter gene. Using the two transformation systems (PEG and electroporation) revealed the efficiency of both, confirmed by fluorescence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Colletotrichum gloeosporioides; Fluorescent proteins; Pathogenicity. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300050 |
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