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In silico evaluation of the Eucalyptus transcriptome Genet. Mol. Biol.
Vicentini,Renato; Sassaki,Flávio T.; Gimenes,Marcos A.; Maia,Ivan G.; Menossi,Marcelo.
The expressed sequence tags (ESTs) produced in the Forests project provide an invaluable opportunity to assess the Eucalyptus transcriptome. Besides providing information on the different proteins produced by this plant, it is possible to infer gene expression profiles because non-normalized cDNA libraries were used. The EST frequency from any gene is correlated to the transcript levels in the tissues from which the cDNA libraries were constructed. The goal of this work was to identify Eucalyptus genes that showed either differential expression pattern or were ubiquitously expressed in the tissues sampled in the Forests project. Six robust statistical tests and very restrictive rules were applied to gain confidence in the in silico data aiming to avoid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transcriptome; Eucalyptus; Tissue-specific; Statistics; Differential expression.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400002
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Eucalyptus ESTs involved in the production of 9-cis epoxycarotenoid dioxygenase, a regulatory enzyme of abscisic acid production Genet. Mol. Biol.
Guerrini,Iraê A.; Trigueiro,Rodrigo M.; Leite,Regina M.; Wilcken,Carlos F.; Velini,Edivaldo D.; Mori,Edson S.; Furtado,Edson L.; Marino,Celso L.; Maia,Ivan G..
Abscisic acid (ABA) regulates stress responses in plants, and genomic tools can help us to understand the mechanisms involved in that process. FAPESP, a Brazilian research foundation, in association with four private forestry companies, has established the FORESTs database (<A HREF="https://forests.esalq.usp.br">https://forests.esalq.usp.br</A>). A search was carried out in the Eucalyptus expressed sequence tag database to find ESTs involved with 9-cis epoxycarotenoid dioxygenase (NCED), the regulatory enzyme for ABA biosynthesis, using the basic local BLAST alignment tool. We found four clusters (EGEZLV2206B11.g, EGJMWD2252H08.g, EGBFRT3107F10.g, and EGEQFB1200H10.g), which represent similar sequences of the gene that produces NCED. Data...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Eucalyptus EST; Abscisic acid; Water deficit; Drought resistance; NCED.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400020
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N-glycosylation in sugarcane Genet. Mol. Biol.
Maia,Ivan G.; Leite,Adilson.
The N-linked glycosylation of secretory and membrane proteins is the most complex posttranslational modification known to occur in eukaryotic cells. It has been shown to play critical roles in modulating protein function. Although this important biological process has been extensively studied in mammals, much less is known about this biosynthetic pathway in plants. The enzymes involved in plant N-glycan biosynthesis and processing are still not well defined and the mechanism of their genetic regulation is almost completely unknown. In this paper we describe our first attempt to understand the N-linked glycosylation mechanism in a plant species by using the data generated by the Sugarcane Expressed Sequence Tag (SUCEST) project. The SUCEST database was...
Tipo: Info:eu-repo/semantics/article
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100030
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Caracterização de um isolado do Sugarcane mosaic virus que quebra a resistência de variedades comerciais de cana-de-açúcar Trop. Plant Pathol.
Gonçalves,Marcos C.; Santos,Amaury S.; Maia,Ivan G.; Chagas,César M.; Harakava,Ricardo.
Os vírus pertencentes ao subgrupo do Sugarcane mosaic virus (SCMV, gênero Potyvirus, família Potyviridae) infectam e causam mosaico em diferentes espécies botânicas da subfamília Panicoideae (família Poaceae), porém apenas o SCMV e o Sorghum mosaic virus (SrMV) infectam naturalmente cana-de-açúcar. No Brasil, a espécie SCMV parece ser o único agente causal da doença. Embora a maioria das variedades comerciais de cana-de-açúcar seja considerada resistente ou tolerante ao SCMV, relatos de incidência de mosaico em tais variedades têm ocorrido no campo. Amostras com sintomas, de diversos clones e variedades, foram coletadas em campos experimentais e comerciais de cana-de-açúcar. Dentre as variedades, a RB72-454, uma das mais plantadas no país e considerada...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Potyviridae; Mosaico da cana; Sugarcane mosaic virus subgroup.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000100004
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