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Comparison between qualitative and semiquantitative catheter-tip cultures: laboratory diagnosis of catheter-related infection in newborns BJM
Marconi,Camila; Cunha,Maria de Lourdes RS; Lyra,João C; Bentlin,Maria R; Batalha,Jackson EN; Sugizaki,Maria Fátima; Rugolo,Lígia MSS.
This prospective study evaluated semiquantitative and qualitative catheter-culture methods for diagnosis of catheter-related infection (CRI) in newborns. Catheter tips from newborns admitted to the Neonatal Unit of the University Hospital of the Botucatu Medical School, UNESP were included in the study. Catheter cultures were performed with both semiquantitative and qualitative techniques. For CRI diagnosis, microorganisms isolated from catheter cultures and from peripheral blood cultures were identified and submitted to agent susceptibility test. The gold standard was the certain CRI diagnosis when same microorganism (specie and profile of susceptibility to agents) was isolated from both catheter tips and peripheral blood culture. A total of 85 catheters...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catheter-related infection; Catheter culture; Semiquantitative culture; Newborns; Coagulase-negative staphylococci.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200012
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Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates BJM
Brolazo,Eliane Melo; Leite,Domingos Silva; Tiba,Monique Ribeiro; Villarroel,Marina; Marconi,Camila; Simoes,Jose Antonio.
Identification of Lactobacillus sp. strains by phenotypic methods may lead to doubtful results possibly interfering in the reliability of the epidemiological and probiotics studies. Therefore this study aimed to determine the best methodology for the identification of the large diversity of lactobacilli species found in the vagina by comparing two techniques, one based on their biochemical profile and other employing molecular biology. A carbohydrate fermentation test (API 50 CH) was compared with multiplex polymerase chain reaction (PCR) for the identification of species of vaginal lactobacilli from 135 healthy women. The kappa index was used to evaluate agreement between the methods. Using the molecular technique, L. crispatus (32.6%), L. jensenii (25%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrate fermentation test; Multiplex PCR; Identification; Vaginal lactobacilli; Vaginal ecosystem.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100028
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