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| Mari, Jocelyne; Bonami, Jean-robert; Lightner, Donald V. |
| The ssRNA genome extracted from purified Taura Syndrome Virus (TSV) was transcribed into double-stranded, blunt-ended cDNA and was used to construct cDNA libraries either in pUC 18 or in pBluescript II KS-vectors. Twelve recombinant plasmids chosen after screening of the libraries were subjected to restriction enzyme digestions for determination of size inserts and restriction maps. Two of them, pP15 and pQ1, were selected for probe construction. The inserts, 1500 and 1300 base pairs (bp) respectively, were DIG-11dUTP-labelled and the corresponding probes were named P15 and Q1. On northern blots and dot blots, using different denaturation methods, the 2 probes hybridized specifically with extracted RNA-TSV genome, TSV and infected TS shrimp homogenates. No... |
| Tipo: Text |
Palavras-chave: TSV; Penaeid shrimp; Picornavirus; Cloning; Dot blot hybridization; In situ hybridization. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00447/55899/57914.pdf |
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| Comps, Michel; Mari, Jocelyne; Poisson, Francois; Bonami, Jean-robert. |
| A cytoplasmic dsRNA virus, rotifer birnavirus (RBV), has recently been isolated from the rotifer Brachionus plicatilis and is associated with a high mortality rate. Histologically, the viral lesions consist of characteristic inclusions, particularly amorphous dense bodies containing occluded particles. Purified virions are about 59 nm in diameter, single-shelled and display four capsomers per edge. The purified virions have a buoyant density of 1.290 (full particles) and 1.250 (empty particles) in CsCI gradients. Four major structural polypeptides of Mrs 60K, 52K, 33K and 27K were detected by SDS-PAGE. The genome is composed of two linear segments of dsRNA with Mrs of 2.45 x 106 and 2.31 × 106; additionally, small circular ssRNA molecules were detected by... |
| Tipo: Text |
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| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00129/24032/21994.pdf |
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| Mari, Jocelyne; Poulos, Bonnie; Lightner, Donald; Bonami, Jean-robert. |
| The single-stranded genomic RNA of Taura syndrome virus (TSV) is 10205 nucleotides in length, excluding the 3' poly(A) tail, and contains two large open reading frames (ORFs) that are separated by an intergenic region of 207 nucleotides. The ORFs are flanked by a 377 nucleotide 5' untranslated region (UTR) and a 226 nucleotide 3' UTR followed by a poly(A) tail. The predicted amino acid sequence of ORF1 revealed sequence motifs characteristic of a helicase, a protease and an RNA-dependent RNA polymerase, similar to the non-structural proteins of several plant and animal RNA viruses. In addition, a short amino acid sequence located in the N-terminal region of ORF1 presented a significant similarity with a baculovirus IAP repeat (BIR) domain of inhibitor of... |
| Tipo: Text |
Palavras-chave: Genomic characterization; Taura syndrome; Virus; Shrimp disease; Penaeidae. |
| Ano: 2002 |
URL: https://archimer.ifremer.fr/doc/2002/publication-715.pdf |
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