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| Marti, Romain; Ribun, Sebastien; Aubin, Jean-baptiste; Colinon, Celine; Petit, Stephanie; Marjolet, Laurence; Gourmelon, Michele; Schmitt, Laurent; Breil, Pascal; Cottet, Marylise; Cournoyer, Benoit. |
| Rivers are often challenged by fecal contaminations. The barrier effect of sediments against fecal bacteria was investigated through the use of a microbial source tracking (MST) toolbox, and by Next Generation Sequencing (NGS) of V5-V6 16S rRNA gene (rrs) sequences. Non-metric multi-dimensional scaling analysis of V5-V6 16S rRNA gene sequences differentiated bacteriomes according to their compartment of origin i.e., surface water against benthic and hyporheic sediments. Classification of these reads showed the most prevalent operating taxonomic units (OTU) to be allocated to Flavobacterium and Aquabacterium. Relative numbers of Gaiella, Haliangium, and Thermoleophilum OTU matched the observed differentiation of bacteriomes according to river compartments.... |
| Tipo: Text |
Palavras-chave: Peri-urban river; Benthic and hyporheic sediments; Microbial community; High throughput sequencing (HTS); Fecal contamination. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00378/48886/49316.pdf |
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