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Aplicaciones de la Biotecnología en la Salud y Mejora Animal Rev Salud Anim.
Martínez Marrero,Siomara.
Tipo: Journal article
Ano: 2012 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000300015
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Influence of culture medium components on Mycoplasma gallisepticum growth Rev Salud Anim.
Pérez Bueno,Tania; Sánchez Miranda,Lilian; Rosado Ruiz-Apodaca,Ileana; Díaz Bravo,Tito; Rodríguez Edreira,Mercedes; Martínez Marrero,Siomara.
The composition of a culture medium produced in Cuba to grow Mycoplasma gallisepticum was evaluated using a response surface methodology. Initially, the influence of Mycoplasma base, horse serum and yeast extract on biomass production was assessed in a 2³ factorial experimental design. Then, a central composite design was applied. Mycoplasma base and horse serum factors were selected for this study. In 7 L and 35 L bioreactors, the variations carried out in the culture medium composition were estimated. The experimental results were fitted to a second order polynomial model equation. Finally, the optimal growth conditions established were at concentrations of 1,88% Mycoplasma base and 18,16% horse serum (v/v) with 0,6% of yeast extract. For the conditions...
Tipo: Journal article Palavras-chave: Culture medium optimization; Mycoplasma gallisepticum; Statistical experimental design.
Ano: 2014 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2014000200009
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Cell surface characteristics and adherence of typeable and non-typeable strains of Streptococcus suis from pig farms in Cuba Rev Salud Anim.
Espinosa Castaño,Ivette; Arias Baéz,Michel; Lobo Rivero,Evelyn; Martínez Marrero,Siomara.
Streptococcus suis infection is considered a major problem in the swine industry. There are 35 known serotypes of S. suis based on the capsular polysaccharides (CPS) on the cell surface. Serotype 2 is the most virulent in pigs and humans. Some S. suis strains do not agglutinate with any of the typing antisera and are identified as non-typable strains, which have generally been viewed as organisms that do not cause important clinical infections. Previous studies have shown differences in the composition and properties of the cell surface among these strains. The aim of this study included the characterization of cell hydrophobicity, ability to adhere to plates, and autolysis over time of typeable and non-typeable strains of S. suis from farms of the Cuban...
Tipo: Journal article Palavras-chave: Streptococcus suis; Hydrophobicity; Adherence; Autolysine.
Ano: 2015 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000300007
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Identificación de los genes que codifican para las proteínas VirB9, VirB10, proteína conjugal de transferencia y el factor de elongación -Tu de un aislamiento cubano de Anaplasma marginale Rev Salud Anim.
Corona Gonzalez,Belkis; Martínez Marrero,Siomara; Massard,Carlos Luiz; Da Fonseca,Adivaldo Henrique; Sandes Pires,Marcus; Peckle Peixoto,Maristela; Bezerra Da Silva,Claudia; Obregón Rodríguez,Dasiel; Díaz Sánchez,Adrian; Azevedo Santos,Huarrisson.
A partir de la secuenciación del genoma completo de aislamientos de Anaplasma marginale, se ha logrado la identificación de genes que codifican para proteínas de membrana de este hemoparásito y la identificación de nuevos inmunógenos, como posibles candidatos vacunales. Sin embargo, se conoce poco acerca de la variabilidad genética de genes que codifican para proteínas de membrana de aislamientos de Anaplasma marginale. El objetivo del presente estudio fue determinar el grado de conservación en la secuencia nucleotídica de los genes que codifican para las proteínas VirB9, VirB10, la proteína conjugal de transferencia (CTP) y el factor de elongación -Tu (Ef-Tu) de un aislamiento cubano de Anaplasma marginale, así como de la secuencia deducida de...
Tipo: Journal article Palavras-chave: Anaplasma marginale; VirB9; VirB10; CTP; Ef-Tu.
Ano: 2015 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000100002
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