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| Santerre, Christelle; Sourdaine, Pascal; Marc, Nicolas; Mingant, Christian; Robert, Rene; Martinez, Anne-sophie. |
| The sex-determining system of Crassostrea gigas is still poorly known, especially regarding the potential influence of temperature. In order to address this question, mRNA expressions of actors of the molecular cascade (Cg-DMI, Cg-SoxE, Cg-beta-catenin, Cg-Foxl2/Cg-FoxI2os) and of Oyvlg, a germ cell marker, were investigated by real-time PCR in spat grown at different temperatures (18, 22, 25 and 28 degrees C). In parallel, gonadic differentiation, gametogenesis and sex ratios were assessed by histology at each of these temperatures. Whatever the temperature, Cg-DMI, Cg-SoxE, Cg-beta-catenin and Oyvlg expressions peaked at the same developmental stage, always after Cg-Foxl2/Cg-Foxl2os (around 40-44 dpf for spat grown at 18 degrees C). Temperatures... |
| Tipo: Text |
Palavras-chave: Oyster spat; Temperature; Sex ratio; TSD; Sex determination pathway. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00136/24767/22874.pdf |
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| Broquard, Coralie; Martinez, Anne-sophie; Maurouard, Elise; Lamy, Jean-baptiste; Dégremont, Lionel. |
| Understanding sex determination in the Pacific oyster Crassostrea gigas, a sequential hermaphrodite, can provide prospective on the evolution of sex-determining systems for comparative reproduction from an evolutionary perspective. Surprisingly, this mechanism is still poorly understood. To date, sex ratio and sex change have never been studied at the individual level for a large size group and long-term monitoring. To this purpose, we performed an ambitious individual long-term follow-up (6 years) on a large population (cohort 1: 7488 oysters) produced from wild oysters, as well as for a second population produced from the cohort 1 (cohort 2: 4320 oysters). All oysters were individually sexed from 2014 to 2019. For the cohort 1, our results showed a... |
| Tipo: Text |
Palavras-chave: Sex-ratio; Sex change; Oysters; Hermaphroditism; Crassostrea gigas. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00587/69868/67845.pdf |
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