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Deep Sea Microbes Probed by Incoherent Neutron Scattering Under High Hydrostatic Pressure ArchiMer
Peters, Judith; Martinez, Nicolas; Michoud, Gregoire; Carlo, Anais; Franzetti, Bruno; Oger, Philippe; Jebbar, Mohamed.
The majority of the biosphere is a high pressure environment. Around 70% of the marine biosphere lies at depths below 1000 m, i.e. at pressures of 100 bars or higher. To survive in these environments, deep-biosphere organisms have adapted to life at high pressure. In vitro studies showed that the activity of certain proteins originating from deep-sea organisms is less affected by high pressure than that of enzymes from surface organisms [1-3]. However, the genetic and structural bases for this increased pressure resistance are still unknown. Elastic incoherent neutron scattering studies, which provide access to information about molecular dynamics, constitute a very promising approach to decipher the structural adaptation in proteins living under high...
Tipo: Text Palavras-chave: Neutron Scattering; Molecular Dynamics; High Pressure; Deep Sea Microbes.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00251/36186/34725.pdf
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The Effect of Crowding on Protein Stability, Rigidity, and High Pressure Sensitivity in Whole Cells ArchiMer
Golub, Maksym; Martinez, Nicolas; Michoud, Gregoire; Ollivier, Jacques; Jebbar, Mohamed; Oger, Philippe; Peters, Judith.
In live cells, high concentrations up to 300–400 mg/mL, as in Eschericia coli (Ellis, R. J. Curr. Opin. Struct. Biol. 2001, 11, 114) are achieved which have effects on their proper functioning. However, in many experiments only individual parts of the cells as proteins or membranes are studied in order to get insight into these specific components and to avoid the high complexity of whole cells, neglecting by the way the influence of crowding. In the present study, we investigated cells of the order of Thermococcales, which are known to live under extreme conditions, in their intact form and after cell lysis to extract the effect of crowding on the molecular dynamics of the proteome and of water molecules. We found that some parameters characterizing the...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00454/56526/58329.pdf
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Habitat structure versus food abundance: the importance of sparse vegetation for the common redstart Phoenicurus phoenicurus Organic Eprints
Martinez, Nicolas; Jenni, Lukas; Wyss, Eric; Zbinden, Niklaus.
As many other birds breeding in agricultural areas, the common redstart declined strongly in many Central European countries over the last 60 years. The destruction of traditionally managed orchards, an important breeding habitat in Central Europe, is a relevant cause. An additional factor for the decline of this species could be the intensified management of the ground vegetation in orchards through reducing food availability and lowering prey detectability and accessibility. In this study we examined the importance of surfaces with sparse vegetation for the location of redstart territories and for foraging. To validate the results of these field studies we made habitat-choice experiments in aviaries with captive birds. Territories occupied by redstarts...
Tipo: Journal paper Palavras-chave: Biodiversity and ecosystem services; Fruit and berries.
Ano: 2010 URL: http://orgprints.org/16970/1/Martinez_etal_2010_JOrn_Phopho.pdf
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