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Ferreira Neto,José Mauricio; Ferreira,Fernanda Pinto; Miura,Ana Carolina; Almeida,Jonatas Campos de; Martins,Felippe Danyel Cardoso; Souza,Marielen de; Bronkhorst,Dalton Evert; Romanelli,Paulo Roberto; Pasquali,Aline Kuhn Sbruzzi; Santos,Hannah Lia Ettiene Peruch Lemos dos; Benitez,Aline do Nascimento; Caldart,Eloiza Teles; Zanella,Luiz Francisco; Freire,Roberta Lemos; Navarro,Italmar Teodorico. |
ABSTRACT: The present study aimed to investigate an abortion outbreak in a dairy goat herd in the municipality of Arapoti, Parana, Brazil. At the beginning of the outbreak, blood samples were collected from 33 goats with clinical signs; later, of the whole goat herd, two cats and two dogs. Milk samples were collected from 78 lactating goats. Four environmental soil samples and four samples of feed residue from goat feeders were collected too. Immunofluorescence antibody test (IFA) was used for serodiagnosis, the molecular analysis was conducted by means of the polymerase chain reaction (PCR), for the isolation of the etiological agent the bioassay was used. The results of the IFA revealed that 76.53% (137/179) of the goats, two dogs and two cats were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasma; Goats. IFA; PCR; Bioassay.. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000500500 |
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Guerra,Joice Loures; Okano,Werner; Bogado,Alexey Leon Gomel; Nino,Beatriz de Souza Lima; Martins,Felippe Danyel Cardoso; Cardim,Sérgio Tosi; Barros,Luiz Daniel de; Garcia,João Luis. |
ABSTRACT: The presence of anti-Neospora caninum antibodies in beef cattle slaughtered in the northern region of the state of Paraná, Brazil, was evaluated. A total of 401 blood samples were collected; 281 samples from the municipality of Rolândia and 120 from the municipality of Borrazópolis, between April 2015 and November 2016. Of the total samples, 289 were from females and 112 from males, aged one and a half to eight years. Indirect fluorescent antibody test (IFAT) was performed, using a cut-off of 1:100. Variables were tabulated for statistical analyses (Fisher’s exact test and chi-square tests, p≤0.05). The analysis showed that of the 401 samples, 37 were positive for N. caninum, indicating a prevalence of 9.2 %, and observed titers were 1:100 (16),... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neosporosis; Seroprevalence; IFAT; Cattle; Paraná state. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500500 |
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