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	Provedor de dados BJM (2) Autor Martins,Maurilio L. (2) Vanetti,Maria C.D. (2) Araújo,Elza F. de (1) Mantovani,Hilário C. (1) Pinto,Uelinton M. (1) Riedel,Katharina (1) Riedel,Kathrin (1) Uelinton,M. Pinto (1) Mais... Palavra-chave Pseudomonas fluorescens (2) Biofilm formation (1) Extracellular lipase (1) Extracellular protease (1) Food deterioration (1) Milk (1) Quorum quenching (1) Quorum sensing (1) Raw milk (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2015 (1) 2014 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk BJM Martins,Maurilio L.; Uelinton,M. Pinto; Riedel,Kathrin; Vanetti,Maria C.D.; Mantovani,Hilário C.; Araújo,Elza F. de. Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming... Tipo: Info:eu-repo/semantics/article Palavras-chave: Milk; Pseudomonas fluorescens; Biofilm formation; Quorum sensing; Quorum quenching. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300037 Milk-deteriorating exoenzymes from Pseudomonas fluorescens 041 isolated from refrigerated raw milk BJM Martins,Maurilio L.; Pinto,Uelinton M.; Riedel,Katharina; Vanetti,Maria C.D.. The practice of refrigerating raw milk at the farm has provided a selective advantage for psychrotrophic bacteria that produce heat-stable proteases and lipases causing severe quality problems to the dairy industry. In this work, a protease (AprX) and a lipase (LipM) produced by Pseudomonas fluorescens 041, a highly proteolytic and lipolytic strain isolated from raw milk obtained from a Brazilian farm, have been purified and characterized. Both enzymes were purified as recombinant proteins from Escherichia coli. The AprX metalloprotease exhibited activity in a broad temperature range, including refrigeration, with a maximum activity at 37 °C. It was active in a pH range of 4.0 to 9.0. This protease had maximum activity with the substrates casein and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Raw milk; Food deterioration; Pseudomonas fluorescens; Extracellular protease; Extracellular lipase. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100207 Registros recuperados: 2 Primeira ... 1 ... Última

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