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Molecular detection of virulence factors among food and clinical Enterococcus faecalis strains in South Brazil BJM
Medeiros,A.W.; Pereira,R.I.; Oliveira,D.V.; Martins,P.D.; d'Azevedo,P.A.; Van der Sand,S.; Frazzon,J.; Frazzon,A.P.G.
The present report aimed to perform a molecular epidemiological survey by investigating the presence of virulence factors in E. faecalis isolated from different human clinical (n = 57) and food samples (n = 55) in Porto Alegre, Brazil, collected from 2006 to 2009. In addition, the ability to form biofilm in vitro on polystyrene and the β-haemolytic and gelatinase activities were determined. Clinical strains presented a higher prevalence of aggregation substance (agg), enterococcal surface protein (esp) and cytolysin (cylA) genes when compared with food isolates. The esp gene was found only in clinical strains. On the other hand, the gelatinase (gelE) and adherence factor (ace) genes had similar prevalence among the strains, showing the widespread...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Virulence determinants; Clinical enterococci; Food enterococci; Biofilm formation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100047
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Biofilm formation on polystyrene under different temperatures by antibiotic resistant Enterococcus faecalis and Enterococcus faecium isolated from food BJM
Marinho,A.R.; Martins,P.D.; Ditmer,E.M.; d'Azevedo,P.A.; Frazzon,J.; Sand,S.T.Van Der; Frazzon,A.P.G..
The ability of antibiotic resistant E. faecalis and E. faecium isolated from food to form biofilm at different temperatures in the absence or presence of 0.75% glucose was evaluated. A synergistic effect on biofilm at 10 °C, 28 °C, 37 °C and 45 °C and glucose was observed for E. faecalis and E. faecium.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococci; Resistant; Food; Temperatures; Biofilm; Polystyrene.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200012
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