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Mello,R.H.; Martins,R.F.; Bonissi,D.A.; Júnior,J.L.B.; Melotti,T.; Bianchi,H.; Bianchi,M.A.F.. |
ABSTRACT To conduct the survey were used 35 (thirty-five) red-browned parrots (A. rhodocorytha), adults, captive, of both genders and clinically healthy, belonging to the live collection of the Museum of Biology Teacher Mello Leitao, located in Santa Teresa, Espírito Santo, Brazil. Harvests were performed in the morning, by puncture of the brachial vein getting 0.5mL of blood stored in EDTA for a period no longer than 6 hours. Blood smears of fresh material were made at collection, stained using the method of May-Grunwald-Giemsa. Analysis of blood elements was done by cell counting in a mirrored Neubauer chamber using Natt and Herrick solution at a ratio of 2:200 as diluent. For the analysis of the methodology, homoglobinometry cyanide hemoglobin using... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wild birds; Hematology; Blood count; Parrots; Clinical pathology. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501275 |
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Santos,F.G.A.; Vasconcelos,A.C.; Nunes,J.E.S.; Cassali,G.D.; Paixão,T.A.; Martins,A.S.; Silva,S.S.; Martins,R.F.; Moro,L.. |
Twelve male, mongrel, adult dogs were subcutaneously transplanted with cells originated from two canine transmissible venereal tumors (TVT). The aim was to demonstrate and to quantify the occurrence of apoptosis in the TVT regression. After six months of transplantation, a tumor sample was obtained from each dog, being six dogs with TVT in the growing phase and six in the regression phase as verified by daily measurements. Samples were processed for histological and ultrastructural purposes as well as for DNA extraction. Sections of 4µm were stained by HE, Shorr, methyl green pyronine, Van Gieson, TUNEL reaction and immunostained for P53. The Shorr stained sections went through morphometry that demonstrated an increase of the apoptotic cells per field in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Venereal tumor; Apoptosis; Cell death. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300013 |
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