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Purification and Characterization of a Polygalacturonase Produced by Wickerhamomyces anomalus BABT
Martos,María Alicia; Butiuk,Ana Paula; Rojas,Natalia Lorena; Hours,Roque Alberto.
The aim of this work was to study the purification and physicochemical properties of an endo-polygalacturonase (PG) produced by Wickerhamomyces anomalus isolated from the citrus fruit peels. The enzyme was purified to homogeneity from the culture filtrate of W. anomalus grown on the yeast nitrogen base medium with glucose as carbon and energy source and citrus pectin as inductor. After anion-exchange chromatography and gel filtration chromatography, PG activity was eluted as a single peak, yielding 21% of the original activity. After dialysis and cation-exchange chromatography, only one fraction with PG activity was obtained, recovering 56% of initial enzyme activity and 1.3-fold increase in specific activity. The molecular weight of the enzyme was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wickerhamomyces anomalus; Polygalacturonase; Purification; Characterization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400587
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