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Molecular weight estimation of esterase isoenzymes in closely related Drosophila Species (Diptera: Drosophilidae) in non-denaturing polyacrylamide gel electrophoresis BABT
Mateus,Rogério Pincela; Cabral,Hamilton; Bonilla-Rodriguez,Gustavo Orlando; Ceron,Carlos Roberto.
A method that allows the measure of molecular weight of two well-known and closely related esterases from Drosophila mojavensis and its sibling species, D. arizonae, is here described, using native polyacrylamide gel electrophoresis at several concentrations, applying Fergunson´s principles. These enzymes, namely EST-4 and EST-5, presented molecular weight values between 81 and 91 kDa. In spite of their distinct expression pattern through the insect's life cycle, they showed properties of isoenzymes codified by distinct structural genes, supporting the hypothesis of a rather recent gene duplication event that generated both in D. mojavensis and D. arizonae, as well as in other species of repleta group. The method is simple and adequate to be applied to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Esterases; Isoenzymes; Molecular weight determination; Drosophila mojavensis; Drosophila arizonae; PAGE.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500004
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Adult sex ratio effects on male survivorship of Drosophila melanogaster Meigen (Diptera, Drosophilidae) Rev. Bras. entomol.
Costa,Marcelo; Mateus,Rogério Pincela; Moura,Mauricio Osvaldo; Machado,Luciana Paes de Barros.
The behavioral biology has a central role in evolutionary biology mainly because the antagonistic relations that occur in the sexual reproduction. One involves the effect of reproduction on the future life expectation. In this scenario, changes in male operational sex ratio could lead to an increase in mortality due to costs associated with excessive courtship and mating displays. Thus, this work experimentally altered the male sex ratio of Drosophila melanogaster Meigen, 1830, to determine its impact on mortality. The results indicated that mortality increases as the sex ratio changes, including modifications in the survivorship curve type and in the curve concavity, measured by entropy.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adult sex ratio; Entropy; Survivor.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262010000300015
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