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| Mazzi,Carmen Maria; Ferro,Jesus Aparecido; Ferro,Maria Inês Tiraboschi; Savino,Vicente José Maria; Coelho,Antonio Augusto Domingos; Macari,Marcos. |
| The promoter region and the beginning of the coding region of the hsp70 stress gene were analysed in broiler chickens of a commercial breed (Hubbard-Pettersen), a breed selected for weight gain (PP1) and a non-selected breed (naked-neck Label Rouge). The naked neck gene (Naked neck, Na), which reduces feathering in birds and is thus related to heat resistance, was present in both PP1 and Label Rouge breeds. Genomic DNA was restricted with PstI and Southern blotting analysis of the samples revealed the absence of polymorphic sites for that enzyme in the promoter region and beginning of the coding region of the hsp70 gene of studied birds. PCR-SSCP analysis of these regions, however, indicated the presence of polymorphisms in the beginning of the coding... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler chickens; Hsp70 gene; Naked neck (Na) gene; Polymorphism; SSCP. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300010 |
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