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| Santana,Priscila Bessa; Ghilardi Junior,Rubens; Alves,Claudio Nahum; Silva,Jeronimo Lameira; McCulloch,John Anthony; Schneider,Maria Paula Cruz; Silva,Artur da Costa da. |
| Methanogenic archaeans are organisms of considerable ecological and biotechnological interest that produce methane through a restricted metabolic pathway, which culminates in the reaction catalyzed by the Methyl-coenzyme M reductase (Mcr) enzyme, and results in the release of methane. Using a metagenomic approach, the gene of the a subunit of mcr (mcrα) was isolated from sediment sample from an anoxic zone, rich in decomposing organic material, obtained from the Tucuruí hydroelectric dam reservoir in eastern Brazilian Amazonia. The partial nucleotide sequences obtained were 83 to 95% similar to those available in databases, indicating a low diversity of archaeans in the reservoir. Two orders were identified -the Methanomicrobiales, and a unique Operational... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazonian; Archaea; Diversity; Methyl-coenzyme M reductase; Mcrα; Homology modeling. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100018 |
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| Reinert,Cristina; McCulloch,John Anthony; Watanabe,Shinya; Ito,Teruyo; Hiramatsu,Keiichi; Mamizuka,Elsa Masae. |
| Methicillin-resistant Staphylococcus aureus (MRSA) commonly causes infection in hospitalized patients. Since its appearance in the 1960s, the SCCmec has evolved throughout the years into 5 different types (I-V), each bearing a different set of genes. Infection with MRSA SCCmec types I, II or III is almost exclusively restricted to hospitalised patients. However, recently, community acquired MRSA (CA-MRSA) infections have been reported with increasing frequency, usually caused by a type IV SCCmec MRSA in nosocomial settings. We studied the prevalence of SCCmec types in 50 nosocomial strains collected from 1995 to 1999. The SCCmec complex type and presence of Panton-Valentine leukocidin (PVL) were determined by PCR. Strains had been previously typed by PFGE... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nosocomial infections; Type IV SCCmec; Staphylococcus aureus; Methicillin-resistant; Brazil. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300010 |
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