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Registros recuperados: 15 | |
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Cleff,Marlete Brum; Meinerz,Ana Raquel; Xavier,Melissa; Schuch,Luiz Filipe; Meireles,Mário Carlos Araújo; Rodrigues,Maria Regina Alves; Mello,João Roberto Braga de. |
The aim of this study was to evaluate the in vitro activity of the essential oil extracted from Origanum vulgare against sixteen Candida species isolates. Standard strains tested comprised C. albicans (ATCC strains 44858, 4053, 18804 and 3691), C. parapsilosis (ATCC 22019), C. krusei (ATCC 34135), C. lusitaniae (ATCC 34449) and C. dubliniensis (ATCC MY646). Six Candida albicans isolates from the vaginal mucous membrane of female dogs, one isolate from the cutaneous tegument of a dog and one isolate of a capuchin monkey were tested in parallel. A broth microdilution technique (CLSI) was used, and the inoculum concentration was adjusted to 5 x 10(6) CFU mL-1. The essential oil was obtained by hydrodistillation in a Clevenger apparatus and analyzed by gas... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida; Origanum vulgare; Essential oil; In vitro. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100018 |
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Albano,Ana Paula Neuschrank; Klafke,Gabriel Baracy; Brandolt,Tchana Martinez; Da Hora,Vanusa Pousada; Nogueira,Carlos Eduardo Wayne; Xavier,Melissa Orzechowski; Meireles,Mário Carlos Araújo. |
Paracoccidioides brasiliensis is the etiological agent of the major systemic mycosis in Brazil, called paracoccidioidomycosis. Although the Rio Grande do Sul is considered an endemic area of the disease, there are few studies on the ecology of P. brasiliensis in the state. Therefore, this study aimed to evaluate the infection of P. brasiliensis in horses from the mesoregion of Southwest Riograndense, using these animals as sentinels. Serological techniques, such as double immunodiffusion in agar gel (AGID) and indirect ELISA, were performed to detect the anti-gp43 P. brasiliensis antibody in horses from five different farms in the region of Bagé, RS, Brazil. Serology was performed in 200 Pure Blood English horses up to two years of age that were born and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ELISA; Gp-43; Protein G-peroxidase; Paracoccidioidomycosis. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200513 |
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Gomes,Fabiane R.; Curcio,Bruna R.; Ladeira,Sílvia R.L.; Fernández,Heriberto; Meireles,Mário Carlos Araújo. |
The aim of this study was to evaluate the occurrence of thermophilic Campylobacter species in broiler flocks from 26 small properties located near the campus of Federal University of Pelotas, RS, Brazil. A total of 404 chicken fecal samples were analyzed and after isolation, identification and biotyping was performed according to Lior´s scheme. Twenty one strains (5.2%) of Campylobacter jejuni biotype II were isolated from animals from seven properties (26.9%), with a variation from 3% to 34.6% among the positive properties. We also analyzed twelve C. jejuni isolates by polymerase chain reaction (PCR), using specific primers that generate a fragment of 402 pb. Our study allowed us to observe that chickens raised in non-industrial establishments without... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Campylobacter jejuni; Thermotolerant species; Diarrhea; Chicken. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300032 |
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Brandolt,Tchana Martinez; Klafke,Gabriel Baracy; Gonçalves,Carla Vitola; Bitencourt,Laura Riffel; Martinez,Ana Maria Barral de; Mendes,Josiara Furtado; Meireles,Mário Carlos Araújo; Xavier,Melissa Orzechowski. |
Abstract Vulvovaginal candidiasis (VVC) is an infection of the genital mucosa caused by different species of the genus Candida. Considering the lack of data on this topic in the south of Brazil, this study aimed to assess the prevalence of Candida spp. in the cervical-vaginal mucosa of patients treated at a university hospital in southern Rio Grande do Sul, as well as the etiology and the susceptibility of the isolates against fluconazole, itraconazole, miconazole and nystatin. Samples were collected at the gynecology clinic of the Federal Hospital of the University of Rio Grande, and the isolates were identified using phenotypic and biochemical tests. The susceptibility analysis was performed according to the CLSI M27-A2 protocol. Of the 263 patients... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vulvovaginal candidiasis; Antifungal; Susceptibility; Resistance. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100145 |
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Santin,Rosema; Mattei,Antonella Souza; Waller,Stefanie Bressan; Madrid,Isabel Martins; Cleff,Marlete Brum; Xavier,Melissa Orzechowski; Nobre,Márcia de Oliveira; Nascente,Patrícia da Silva; Mello,João Roberto Braga de; Meireles,Mário Carlos Araújo. |
The oral microbiota of humans and animals is made up of a wide variety of yeasts and bacteria, but microbiota of dogs is not totally described. Although such identification is an important step to establish the etiopathogenesis and adequate therapy for the periodontal disease The aim of this study was to evaluate and correlate oral alterations with the presence of yeasts in oral cavity of female dogs. After clinical evaluation samples from healthy and from dogs with oral diseases were obtained from three different oral sites by swabs, curettes, millimeter periodontal probes and HA membrane tip in cellulose ester. Yeast identification was performed through macroscopic and microscopic colony features and biochemical tests. Dental calculus was the most... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oral microbiota; Yeasts; M. pachydermatis; Candida spp.; Halitosis. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100020 |
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Nascente,Patrícia da Silva; Meinerz,Ana Raquel Mano; Faria,Renata Osório de; Schuch,Luiz Filipe Damé; Meireles,Mário Carlos Araújo; Mello,João Roberto Braga de. |
Thiabendazole, classified as antiparasitic and also used as an antifungal drug, can be found as otological solution indicated for treatment of parasitic and fungal external otitis in small animals. Malassezia pachydermatis is a yeast recognized as a normal inhabitant on the skin and mucous membranes of dogs and cats. However, it is considered an opportunistic agent that causes external otitis and dermatitis in these animals. The aim of this study was to evaluate the in vitro effect of thiabendazole against 51 isolates of M. pachydermatis using the CLSI Broth Microdilution method that has been adapted for this yeast species (NCCLS, 2002). Based on this test, the Minimum Inhibitory Concentrations (MIC) of thiabendazol was calculated. Subsequently, the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Malassezia pachydermatis; Minimum Inhibitory Concentration; Thiabendazole. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200002 |
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Cleff,Marlete Brum; Lima,Alzira Pacheco de; Faria,Renata Osório de; Meinerz,Ana Raquel Mano; Antunes,Tatiana de Ávila; Araújo,Flávia Biasoli de; Nascente,Patrícia da Silva; Nobre,Márcia de Oliveira; Meireles,Mário Carlos Araújo. |
Yeasts are commensal organisms found in the skin, genital and gastrointestinal tracts, and other mucosa in mammalians. Candida spp has been frequently isolated from domestic and sylvan animals. Yeast isolation from vaginal mucus is frequent in women, especially of Candida genus. In canine females, however, studies about vaginal fungal microbiota are rare and the connection with estrous cycle is unknown. The purpose of this study was to isolate yeast 5 of Candida genus from healthy canine females and identify the isolated species, establishing their connection with estrous cycle phases. Two hundred twenty-four samples taken from vaginal mucus of 14 canine females were analyzed in this study. Candida spp was observed in 83 (37%) from these samples; nine... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida spp; Canine females; Estrous cycle. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200018 |
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Gomes,Angelita dos Reis; Cabana,Ângela Leitzke; Osório,Luiza da Gama; Santin,Rosema; Schuch,Isabel Duarte; Serra,Emanoele Figueiredo; Nascente,Patrícia Silva; Meireles,Mário Carlos Araújo. |
Ear infections in cats are uncommon, especially involving yeasts. This report describes the first isolation of the Stephanoascus ciferrii, teleomorph of the Candida genus, in a case of feline otitis in Brazil. The identification and characterization of Stephanoascus ciferrii were confirmed by the Vitek2 System (BioMerieux ®). |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mixed infection; Identification; External otitis; Cat; Stephanoascus ciferrii. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300045 |
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Waller,Stefanie Bressan; Madrid,Isabel Martins; Ferraz,Vanny; Picoli,Tony; Cleff,Marlete Brum; de Faria,Renata Osório; Meireles,Mário Carlos Araújo; de Mello,João Roberto Braga. |
Abstract The study aimed to evaluate the anti-Sporothrix sp. activity of the essential oil of Origanum majorana Linn. (marjoram), its chemical analysis, and its cytotoxic activity. A total of 18 fungal isolates of Sporothrix brasiliensis (n: 17) from humans, dogs and cats, and a standard strain of Sporothrix schenckii (n: 1) were tested using the broth microdilution technique (Clinical and Laboratory Standard Institute - CLSI M27-A3) and the results were expressed in minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC). The MIC50 and MIC90 of itraconazole against S. brasiliensis were 2 µg/mL and 8 µg/mL, respectively, and the MFC50 and MFC90 were 2 µg/mL and >16 µg/mL, respectively, with three S. brasiliensis isolates... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sporotrichosis; Sporothrix schenckii complex; Antifungal resistance; Marjoram; Lamiaceae. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400896 |
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Waller,Stefanie Bressan; Madrid,Isabel Martins; Faria,Renata Osório de; Cleff,Marlete Brum; Mello,João Roberto Braga de; Meireles,Mário Carlos Araújo. |
ABSTRACT Cases of sporotrichosis in humans and animals without satisfactory clinical response have increased, a warning sign of strains resistant to conventional antifungal agents. The urgent search for alternative therapies was an incentive for research on medicinal plants with anti-Sporothrix spp. properties. A bibliographic survey was performed based on scientific papers about in vitro and in vivo antifungal activity of essential oils and extracts of plants in differents solvents against the fungal of the Sporothrix schenckii complex. The study methodology consisted of a literature review in Google Scholar, Science Direct, Pubmed, Bireme and Springer link with papers from 1986 to 2015. We found 141 species of plants that were investigated, of which 100... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sporothrix schenckii complex; Antifungals; Combretaceae/pharmacognosy; Asteraceae/pharmacognosy; Lamiaceae/pharmacognosy; Medicinal plants/antifungal activity. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200221 |
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Xavier,Melissa Orzechowski; Nobre,Márcia de Oliveira; Sampaio Junior,Daiser Paulo; Antunes,Tatiana de Ávila; Nascente,Patrícia da Silva; Sória,Flávia Biasoli de Araújo; Meireles,Mário Carlos Araújo. |
A esporotricose é uma micose subcutânea, causada pelo fungo dimórfico Sporothrix schenckii. O presente estudo descreve a ocorrência de esporotricose felina na cidade de Pelotas (Rio Grande do Sul - Brasil), e a transmissão para um atendente da clínica veterinária, ocasionada pela arranhadura do animal doente. O felino apresentava deformação do espelho nasal e lesões cutâneas, enquanto o humano apresentava uma lesão ulcerada na região metacarpiana dorsal esquerda. Foram colhidas crostas e/ou exsudato das lesões do felino e do atendente para diagnóstico micológico, os quais foram cultivados em ágar Sabouraud dextrose acrescido de cloranfenicol e incubados a 25 e 37°C, durante 10 dias. O isolamento do S. schenckii confirmou o diagnóstico clínico de... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sporothrix schenckii; Gatos; Arranhadura; Micose; Zoonose. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000600047 |
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Registros recuperados: 15 | |
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