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| Florindo,M.I.; Aragão,M.E.F. de; Silva,A.C.M. da; Otoch,M.L.; Melo,D. Fernandes de; Lima,J.A.A.; Lima,M.G. Silva. |
| There is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. Cowpea severe mosaic virus (CPSMV), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (Vigna unguiculata) leaves infected with CPSMV both administered by gavage to Swiss mice induced a humoral immune response. Groups of 10 Swiss mice (2-month-old females) were immunized orally with 10 daily doses of either 50 µg viral capsid protein (boosters of 50 µg at days 21 and 35 after immunization) or 0.6 mg protein of the crude extract (boosters of 0.6 mg at days 21 and 35 after immunization). Anti-CPSMV antibodies were quantified by ELISA in pooled sera... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oral immunization; Plant virus; Plant virus immunogenicity; Cowpea severe mosaic virus. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000700011 |
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