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Viganó, Joselaine; Braccini, Alessandro Lucca; Schuster, Ivan; Menezes, Vanessa Maria Pereira Silva. |
The present study aimed at pyramiding ASR-resistance genes through microsatellite (SSR) marker-assisted selection (MAS) and demonstrating the pyramiding steps. To obtain the first generation of gene pyramiding, crosses were made between introduced plants (PI’s), which have the genes Rpp1, Rpp2, Rpp3, Rpp4, and Rpp5. F1 plants from the initial crosses were intercrossed to obtain plants with the four resistance genes (second pyramiding generation). Plants selected from this second generation were again intercrossed (third pyramiding generation) to increase the number of pyramided genes. For MAS, we used informative SSR markers in each cross. SSR markers were considered informative when the source resistance allele containing the target gene could be followed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phakopsora pachyrhizi; Gene stacking; Marker-assisted breeding; Durable resistance.. |
Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39619 |
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Viganó, Joselaine; Braccini, Alessandro Lucca; Schuster, Ivan; Menezes, Vanessa Maria Pereira Silva. |
The present study aimed at pyramiding ASR-resistance genes through microsatellite (SSR) marker-assisted selection (MAS) and demonstrating the pyramiding steps. To obtain the first generation of gene pyramiding, crosses were made between introduced plants (PI’s), which have the genes Rpp1, Rpp2, Rpp3, Rpp4, and Rpp5. F1 plants from the initial crosses were intercrossed to obtain plants with the four resistance genes (second pyramiding generation). Plants selected from this second generation were again intercrossed (third pyramiding generation) to increase the number of pyramided genes. For MAS, we used informative SSR markers in each cross. SSR markers were considered informative when the source resistance allele containing the target gene could be followed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phakopsora pachyrhizi; Gene stacking; Marker-assisted breeding; Durable resistance.. |
Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39619 |
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