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| De Rosa Jr.,Vicente E.; Nogueira,Fábio T. S.; Menossi,Marcelo; Ulian,Eugênio C.; Arruda,Paulo. |
| Jasmonic acid (JA) and its ester methyl jasmonate (MeJA) are linolenic acid-derived signaling molecules involved in plant development and stress responses. MeJA regulates gene expression at transcription, RNA processing and translation. We investigated the changes in gene expression in sugarcane leaves exposed to MeJA using cDNA arrays. Total RNA isolated at 0, 0.5, 1, 3, 6, and 12 h following MeJA treatment was labeled with alpha-33P-dCTP and hybridized to nylon filters containing 1,536 cDNA clones. A significant increase in gene expression in response to MeJA was detected for both novel and well known stress-related genes, while genes participating in photosynthesis and carbohydrate assimilation were down-regulated. Searches for conserved domains in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CDNA array; Expression profiling; Methyl-jasmonate; Signaling molecules; Sugarcane. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100014 |
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| Gallinari,Rafael Henrique; Coletta,Rafael Della; Araújo,Pedro; Menossi,Marcelo; Nery,Mariana Freitas. |
| Abstract Hemicellulose and cellulose are essential polysaccharides for plant development and major components of cell wall. They are also an important energy source for the production of ethanol from plant biomass, but their conversion to fermentable sugars is hindered by the complex structure of cell walls. The glucuronic acid substitution of xylan (GUX) enzymes attach glucuronic acid to xylan, a major component of hemicellulose, decreasing the efficiency of enzymes used for ethanol production. Since loss-of-function gux mutants of Arabidopsis thaliana enhance enzyme accessibility and cell wall digestion without adverse phenotypes, GUX genes are potential targets for genetically improving energy crops. However, comprehensive identification of GUX in... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: GUX; Sugarcane; Phylogeny; Angiosperms; Biofuels. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100602 |
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| Drummond,Rodrigo D.; Guimarães,Claudia T.; Felix,Juliana; Ninamango-Cárdenas,Fernando E.; Carneiro,Newton P.; Paiva,Edilson; Menossi,Marcelo. |
| Aluminum is one of the major factors that affect plant development in acid soils, causing a substantial reduction in yield in many crops. In South America, about 66% of the land surface is made up of acid soils where high aluminum saturation is one of the main limiting factors for agriculture. The biochemical and molecular basis of aluminum tolerance in plants is far from being completely understood despite a growing number of studies, and in the specific case of sugarcane there are virtually no reports on the effects of gene regulation on aluminum stress. The objective of the work presented in this paper was to prospect the sugarcane expressed sequence tag (SUCEST) data bank for sugarcane genes related to several biochemical pathways known to be involved... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572001000100029 |
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| Ditt,Renata Fava; Gentile,Agustina; Tavares,Rafael Garcia; Camargo,Sandra Rodriguez; Fernandez,Jorge Hernandez; Silva,Marcio Jose da; Menossi,Marcelo. |
| Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp.) is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of those genes, SsNAC23, a member of the NAC family of plant-specific transcription factors, which are induced by low temperature and other stresses in several plant species. The expression of SsNAC23 was induced in sugarcane plants exposed to low temperatures (4ºC). With the aim of further understanding the regulatory network in response to stress, we used the yeast two-hybrid system to identify... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: SsNAC; In situ hybridization cold stress yeast two hybrid. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400010 |
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