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	Provedor de dados Electron. J. Biotechnol. (4) Autor Mercado,Luis (4) Guzmán,Fanny (3) Gallardo,José A (2) Marshall,Sergio H (2) Rojas,Verónica (2) Arenas,Gloria (1) Morales-Lange,Byron (1) Narváez,Edgar (1) Palacios,Claudia (1) Santana,Paula (1) Schmitt,Paulina (1) Valenzuela,Cristián (1) Mais... Palavra-chave Anti-IL-8 (1) Antibody (1) Antimicrobial peptides (1) Biochemical characterization (1) Caspase-3 (1) Fish antibodies (1) Fish apoptosis (1) Fish chemokine (1) Fish immunity (1) Gill tissues (1) In vitro activity (1) Mussels (1) Piscirickettsia salmonis (1) Rainbow trout (1) Salmonid immunity (1) Synthetic peptide epitope (1) TNF-alpha antibody (1) Mais... Tipo do documento Journal article (4) Ano 2012 (3) 2005 (1) País Chile (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Anti-peptide antibodies: A tool for detecting IL-8 in salmonids Electron. J. Biotechnol. Santana,Paula; Palacios,Claudia; Narváez,Edgar; Guzmán,Fanny; Gallardo,José A; Mercado,Luis. Background: Interleukin 8 is a chemokine that is produced by several types of cells, like macrophages and has chemotactic activity in particular on neutrophils, playing a key role during the inflammatory process. It has been demonstrated at the molecular level that this molecule is present and conserved in several vertebrate groups, pointing its importance. Analysis of the amino acid sequence of IL-8, projected from cDNA of Salmo salar, presents homology with the sequences of mammals, poultry and lamprey, indicating the presence of a homologous molecule in higher fish. However, there is no information at protein level, which allows characterizing the regulatory role of this molecule during the immune response in fish. Results: In this work, we designed and... Tipo: Journal article Palavras-chave: Anti-IL-8; Fish antibodies; Fish chemokine; Rainbow trout; Synthetic peptide epitope. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500020 Development of a caspase-3 antibody as a tool for detecting apoptosis in cells from rainbow trout (Oncorhynchus mykiss) Electron. J. Biotechnol. Rojas,Verónica; Guzmán,Fanny; Valenzuela,Cristián; Marshall,Sergio H; Mercado,Luis. Background: Apoptosis is an active cell death process mediated by caspases activation, in which different extrinsic or intrinsic signalling pathways result in direct activation of effector caspases. Caspase-3 is considered to be the most important of the executioner caspases, which cause the morphological and biochemical changes detected in apoptotic cells. Different bacterial and virus pathogens have developed different strategies to survive inside the host and overcome natural protections, one of them is inducing apoptotic death in infected cells. We have demonstrated previously that Piscirickettsia salmonis activates this process in monocytes/macrophages from salmonid RTS11 cell line both by morphological and caspase detection assays; nevertheless,... Tipo: Journal article Palavras-chave: Antibody; Caspase-3; Fish apoptosis; Piscirickettsia salmonis. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500012 Immunological strategy for detecting the pro-inflammatory cytokine TNF-alpha in salmonids Electron. J. Biotechnol. Rojas,Verónica; Morales-Lange,Byron; Guzmán,Fanny; Gallardo,José A; Mercado,Luis. Background: Tumour necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine which exerts a variety of immunological functions in vertebrates. TNF-α has been identified and cloned in a number of teleost fish species; nevertheless, the functions displayed by this cytokine in fishes remain poorly understood, given that the low sequence identity compared to their mammalian counterpart, limit fish TNF-α detection using mammalian antibodies. Then, for fish immune response characterization is fundamental the production of specific fish anti-TNF-α antibody. Results: We have developed a monoespecific antibody against the pro-inflammatory molecule TNF-α of salmonid fish. TNF-α epitope region was identified... Tipo: Journal article Palavras-chave: Fish immunity; Salmonid immunity; TNF-alpha antibody. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500021 Gill tissues of the mussel Mytilus edulis chilensis: A new source for antimicrobial peptides Electron. J. Biotechnol. Mercado,Luis; Schmitt,Paulina; Marshall,Sergio H; Arenas,Gloria. Antimicrobial peptides are small-sized, cationic and amphipathic molecules able to neutralize pathogenic microorganisms. Their antimicrobial effects tie them to mechanisms of immune defense, which is why they have been normally purified from immune cells. We describe an apparently new group of antimicrobial peptides from gill tissues of the mussel Mytilus edulis chilensis. 20 specimens yielded 40 g of gills which produced 16 mg of an enriched fraction with antimicrobial activity as low as 0.045 µg/µl over reference strains. Considering the chemical nature of these molecules we used an acid extraction procedure followed by consecutive cationic exchange and hydrophobic interaction chromatography steps for peptide enrichment. The resulting... Tipo: Journal article Palavras-chave: Antimicrobial peptides; Biochemical characterization; Gill tissues; In vitro activity; Mussels. Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300008 Registros recuperados: 4 Primeira ... 1 ... Última

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