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| Merino-Contreras,María L.; Sánchez-Morales,Froylán; Jiménez-Badillo,María L.; Peña-Marín,Emyr S.; Álvarez-González,Carlos A.. |
| ABSTRACT Digestive proteases were partially characterized in sheepshead juveniles, using biochemical and electrophoretic techniques. Results showed higher activity level of the stomach proteases (2.39 ± 0.02 U mg protein-1) compared to the intestinal proteases (1.6 ± 0.1 U mg protein-1). The activity of trypsin, chymotrypsin, leucine aminopeptidase and carboxypeptidase A was also recorded. The optimum temperature of the stomach proteases was recorded at 45 °C, while for intestinal proteases was recorded at 55 °C. Stomach proteases showed less stability to temperature changes than intestinal proteases. An optimum pH of 2 was recorded for stomach proteases with high stability under acidic conditions, while an optimum pH of 9 was recorded for intestinal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquaculture; Inhibitors; Pepsin; Proteases; Zymogram. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000400206 |
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