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Miramand, Pierre; Bustamante, Paco; Bentley, Daniel; Koueta, Noussithe. |
The developmental changes in the concentration of 8 essential and non-essential heavy metals (Ag, Cd, Cu, Co, Fe, Pb, V, Zn) in the tissues (digestive gland, cuttlebone and whole animal) of the common cuttlefish Sepia officinalis collected in the bay of the river Seine were monitored from the end of the embryogenesis until the adult reproductive stage. Compared to embryos, juveniles after hatching displayed much higher concentrations of Ag, Cu, Fe and Zn, suggesting an efficient incorporation from seawater. Conversely, the amounts of Cd, Pb and V in hatchlings remained constant suggesting that these metals are barely bioavailable for juveniles. Once the juveniles start to feed, the digestive gland appears to play a major role in the storage of all metals.... |
Tipo: Text |
Palavras-chave: Embryogenesis; Sexual maturity; Cephalopods; Detoxification; Bioaccumulation; Trace element. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2330.pdf |
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Bouilly, Karine; Gagnaire, Beatrice; Bonnard, Marc; Thomas-guyon, Helene; Renault, Tristan; Miramand, Pierre; Lapegue, Sylvie. |
Pacific oysters, Crassostrea gigas, are commonly reared in estuaries where they are exposed to anthropogenic pollution. Much research has been made on the toxicity of cadmium to aquatic organisms because the compound recurrently contaminates their environment. Our study examined the influence of cadmium on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in C. gigas at different stages of life. Adults and juveniles were exposed to two different concentrations of cadmium. The first concentration applied was equivalent to a peak value found in Marennes-Oleron bay (Charente-Maritime, France; 50 ng L-1) and the second was 10 times higher (500 ng L-1). Exposure to 50 ng L-1 cadmium caused a significant... |
Tipo: Text |
Palavras-chave: Phenoloxidase; Flow cytometry; Hemocytes; Aneuploidy; Crassostrea gigas; Cadmium. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1687.pdf |
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Kempf, Marc; Cadour, Gwenael; Jeanneret, Hélène; Mear, Yan; Miramand, Pierre; Merceron, Michel. |
This document is a result of an environmental impact study carried out between 1993 and 1995 on the site of a marine trout culture located in the Harbour of Cherbourg, France. It deals with the solid waste of the farm and concems deposition rate, sediment analysis and chemistry (fine fraction < 63 ~m, organic matter, carbon, nitrogen, copper and zinc), bottom living organisms and underwater video monitoring. The impact observed is very moderate and localised. This is mainly due to the strong d)'TIamics and flushing rate of the site. A seperate volume of the same study deals with dissolved waste and concems the water mass (volume 1 : water and pelagos). (Not controlled OCR) |
Tipo: Text |
Palavras-chave: Rade de Cherbourg; Étude biosédimentaire; Impact; Salmoniculture marine; France; Cherbourg; Benthos; Sediment; Environmental impact; Trout; Marine fish farming. |
Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/1997/rapport-3349.pdf |
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Bustamante, Paco; Bertrand, M; Boucaud Camou, E; Miramand, Pierre. |
The subcellular fractionation of the digestive gland cells of the common cuttlefish Sepia officinalis was performed to investigate the distribution of metals between organelles and cytosol and the different cytosolic fractions separated by gel-filtration chromatography. Total metal concentrations vary over 3 orders of magnitude, ranging from dwt for Pb to dwt for Zn. With the exception of Cd, Co, and Cu, metals were mostly bound to the organelles. Whereas no specific organelle compartment was found for Mn, Ph, and Zn, Fe was mainly associated with nucleus, brown body, and "boule" fraction (i.e., 52%) and 44% of the total Ag was contained in the lysosomal and mitochondria enriched fraction. The link of metals with hydrosoluble proteins in the cytosolic... |
Tipo: Text |
Palavras-chave: Sepia officinalis; Chromatography; Metallothionein; Detoxification; Trace element; Metal; Cephalopod. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2532.pdf |
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Bentley, D.; Mear, Y.; Miramand, Pierre; Murat, A.; Guary, J.c.. |
L'aquaculture de poissons (salmonidés, bars et daurades en cages flottantes, turbots en marais) se développe rapidement en France comme dans certains autres pays européens. Afin de préserver au mieux la qualité de l'environnement marin, et donc, en retour, celle des élevages, il est indispensable de bien connaître l'impact que ce type d'élevage peut avoir sur l'environnement. L'influence des élevages de saumons sur les fonds marins a été étudiée en Scandinavie dès la fin des années 80, en Finlande notamment (UOTILA, 1991). Ces études ont montré un enrichissement en zinc (plus de 400 ppm dans le sédiment superficiel situé juste sous les cages, 150 ppm à seulement 20 mètres des cages), en cuivre (120 ppm sous les cages, 20-30 ppm à 20 mètres) et, plus... |
Tipo: Text |
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Ano: 1997 |
URL: http://archimer.ifremer.fr/doc/00081/19223/16818.pdf |
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Rostagno, Bernard; Violleau, Allain; Brun, Céline; Dietrich, Patrice; Fillon, Alain; Goulletquer, Philippe; Guichet, Roger; Kantin, Roger; Legay, Didier; Loubersac, Lionel; Masson, Daniel; Rocher, Gregory; Ryckaert, Mireille; Thomas, Gerard; Fichet, Denis; Guyot, Thierry; Miramand, Pierre; Pigeot, Jacques; Seguignes, Michel. |
Cette étude, qui a rassemblé les compétences des instances de l'Etat chargées de la surveillance du milieu littoral (DDE, DDASS, IFREMER, Université de La Rochelle) a permis de dresser, à la veille de l'an 2000, un bilan de la situation environnementale du littoral de la « mer des Pertuis». Ce bilan, diversifié dans sa présentation, passe en revue les points marquants de "l'état de santé" de l'environnement, qu'il s'agisse de pollutions ciblées (chimiques, bactériologiques) ou d'autres nuisances variées. Il met en exergue les points forts et les points faibles en matière d'environnement littoral, et rappelle les efforts mis en oeuvre par les différentes administrations chargées de sa surveillance. Dans un premier temps, plus de 250 références... |
Tipo: Text |
Palavras-chave: Environnement; Qualité eaux; Littoral; Caractéristiques physiques; Economie; Qualité chimique; Qualité bactériologique; Phycotoxines; Biodiversité; Pollution; Recommandations; Pertuis Charentais; Département Charente-Maritime. |
Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00072/18371/15943.pdf |
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Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Miramand, Pierre; Lapegue, Sylvie. |
Aneuploidy is the alteration of the normal diploid chromosome number. In the Pacifie oyster, Crassastrea gigas, hypodiploid cells have regularly been reported as have a negative correlation between this phenomenon and growth and evidence for a genetic basis. We previously demonstrated a positive relationship between a pollutant, atrazine, and aneuploidy in Crassastrea gigas adults and juveniles. To evaluate the persistence of this impact, the present study focused on a sample of the same juveniles previously exposed to different atrazine treatments (0.0 1 mg/l which represents a peak value found in a polluted environment and 0.1 mg/l) for three and a half months. Then, we evaluated them for aneuploidy after another two and a half months in non polluted... |
Tipo: Text |
Palavras-chave: Cadmium; Atrazine; Pollutant; Pacific oyster; Aneuploidy. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/acte-2852.pdf |
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Faucher, Karine; Fichet, Denis; Miramand, Pierre; Lagardere, Jean-paul. |
The effect of chronic exposure to a low concentration (0.5 mu g l(-1)) of cadmium ions was investigated on escape behaviour of sea bass, Dicentrarchus labrax, using video analysis. Observations were also performed on the microanatomy of lateral system neuromasts. When fish were exposed for 4 h per day over 8 days to the cadmium ions, most of both types of neuromasts observed remained intact. However, some of them presented damaged sensory maculae. Whereas before cadmium exposure, fish responded positively to nearly all the lateral system stimulations, after exposure they decreased by about 10% their positive responses to Stimulations. From the 15th day after the beginning of cadmium exposure, neuromasts presented progressively less damage, cadmium... |
Tipo: Text |
Palavras-chave: Bioaccumulation; Escape behaviour; Chronic cadmium exposure; Neuromast; Fish lateral system. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3939.pdf |
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