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| Melo,Nadja Rodrigues de; Taguchi,Hideaki; Culhari,Vitoria V.P.; Sano,Ayako; Fukushima,Kazutaka; Miyaji,Makoto; Manning,Nigel; Kelly,Steven L.; Vilela,Marluce S.. |
| This study investigated the prevalence of C. dubliniensis in a Brazilian family with an HIV - infected child. A total of 42 oral isolates were obtained from eight family members. The identification of C. dubliniensis was performed by polymerase chain reactions (PCR) using primers against a specific sequence of the C. dubliniensis cytochrome b gene. Only the HIV-infected child and his grandmother were colonized by C. dubliniensis. In this study C. dubliniensis isolated from the HIV-infected child exhibited high susceptibility for azoles tested with MICs of 0.125 and 0.5 µg/mL for voriconazole and fluconazole, respectively. Accumulation of [³H] fluconazole in C. dubliniensis isolated from the HIV-infected child was slightly reduced in comparison to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida dubliniensis; Antifungal agents; Sterol composition. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300008 |
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| Andrade,Vânia Sousa; Sarubbo,Leonie Asfora; Fukushima,Kasutaka; Miyaji,Makoto; Nishimura,Kazuko; Campos-Takaki,Galba Maria de. |
| Recently, some Mucorales species have been reported as protease producers. The production of extracellular proteases by Mucor circinelloides using glucose as substrate was studied. Experiments were carried out with different D-glucose concentrations (40, 60 and 80 g/L). Biomass, pH and protease activity were determined. Although biomass production had reached best yields for the medium containing D-glucose in a concentration of 80 g/L, the enzymatic production was higher when the substrate concentration was reduced to 40 g/L. The yield factor for product on cell growth and the yield factor for product on carbon substrate were higher when the microorganism grew in medium containing 40 g/L glucose. The kinetics parameters suggest that this strain seems to be... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Extracellular proteases; D-glucose; Substrate; Mucor circinelloides. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200002 |
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| Melo,Nadja Rodrigues de; Vilela,Maria Marluce Santos; Jorge Junior,Jacks; Kamei,Katsuhiko; Miyaji,Makoto; Fukushima,Kazutaka; Nishimura,Kazuko; Groeneveld,Philip; Kelly,Steven L.; Taguchi,Hideaki. |
| Declining incidence of oropharyngeal candidosis and opportunistic infections over recent years can be attributed to the use of highly active anti-retroviral therapy (HAART). Infection with C. albicans generally involves adherence and colonization of superficial tissues. During this process, budding yeasts are able to transform to hyphae and penetrate into the deep tissue. Using the biocell tracer system, C. albicans hyphal growth was dynamically observed at the cellular level. Ritonavir was effective in the inhibition of hyphal growth with growth rate of 0.8 mum/min. This study showed the in vitro effect of HIV anti-retroviral drug on the growth rate of the C. albicans hyphae. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida; Hyphal; Protease inhibitor. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300006 |
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